A versatile evaporation-assisted methodology based on the coffee-drop effect is described
to deposit nanoparticles on surfaces, obtaining for the first time patterned gradients of …

Inclusion bodies (IBs) are protein aggregates formed under recombinant protein production
processes in microbial cell factories. Their characterization has shown that they are self …

Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of
novel applications for this type of submicron particulate protein clusters, with potential uses …

The development of genetic engineering techniques has speeded up the growth of the
biotechnological industry, resulting in a significant increase in the number of recombinant …

Bacterial inclusion bodies (IBs) are discrete macromolecular complexes that appear in
recombinant prokaryotic cells under stress conditions. These structures are often discarded …

The physicochemical characterization of protein aggregates yields an important contribution
to further our understanding on many diseases for which the formation of protein aggregates …

Production of protein-based pharmaceuticals is a major issue in conventional
pharmacology, biomedicine and nanomedicine. Being mostly obtained by genetic …

The processing of inclusion bodies (IBs) into surfaces is of great interest for cell culture
applications due to the combined physical and biological cues these particles provide. The …

Homogeneously glycosylated proteins are essential for analyzing the structure of N‐glycans,
studying their consequences inside cells, and developing therapeutic glycoproteins …

Four decades have passed since the first recombinant protein, somatostatin, was produced
in Escherichia coli. To date, more than 160 biopharmaceuticals gained medical approval. As …