Current developments and challenges in plant viral diagnostics: A systematic review

GT Mehetre, VV Leo, G Singh, A Sorokan, I Maksimov… - Viruses, 2021 - mdpi.com
Plant viral diseases are the foremost threat to sustainable agriculture, leading to several
billion dollars in losses every year. Many viruses infecting several crops have been …

A primer on the analysis of high-throughput sequencing data for detection of plant viruses

D Kutnjak, L Tamisier, I Adams, N Boonham… - Microorganisms, 2021 - mdpi.com
High-throughput sequencing (HTS) technologies have become indispensable tools
assisting plant virus diagnostics and research thanks to their ability to detect any plant virus …

Facilitating the adoption of high‐throughput sequencing technologies as a plant pest diagnostic test in laboratories: A step‐by‐step description

B Lebas, I Adams, M Al Rwahnih, S Baeyen… - EPPO …, 2022 - Wiley Online Library
High‐throughput sequencing (HTS) is a powerful tool that enables the simultaneous
detection and potential identification of any organisms present in a sample. The growing …

Small RNA-omics for plant virus identification, virome reconstruction, and antiviral defense characterization

MM Pooggin - Frontiers in microbiology, 2018 - frontiersin.org
RNA interference (RNAi)-based antiviral defense generates small interfering RNAs that
represent the entire genome sequences of both RNA and DNA viruses as well as viroids …

Towards the validation of high-throughput sequencing (HTS) for routine plant virus diagnostics: measurement of variation linked to HTS detection of citrus viruses and …

R Bester, G Cook, JHJ Breytenbach, C Steyn… - Virology Journal, 2021 - Springer
Background High-throughput sequencing (HTS) has been applied successfully for virus and
viroid discovery in many agricultural crops leading to the current drive to apply this …

Characterization of potato virus Y isolates and assessment of nanopore sequencing to detect and genotype potato viruses

M Della Bartola, S Byrne, E Mullins - Viruses, 2020 - mdpi.com
Potato virus Y (PVY) is the most economically important virus infecting cultivated potato
(Solanum tuberosum L.). Accurate diagnosis is crucial to regulate the trade of tubers and for …

Thermotherapy followed by shoot tip cryotherapy eradicates latent viruses and apple hammerhead viroid from in vitro apple rootstocks

JC Bettoni, G Fazio, L Carvalho Costa… - Plants, 2022 - mdpi.com
Virus and viroid-free apple rootstocks are necessary for large-scale nursery propagation of
apple (Malus domestica) trees. Apple stem grooving virus (ASGV) and Apple chlorotic leaf …

Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses

F Pasin, W Menzel, JA Daròs - Plant Biotechnology Journal, 2019 - Wiley Online Library
Recent metagenomic studies have provided an unprecedented wealth of data, which are
revolutionizing our understanding of virus diversity. A redrawn landscape highlights viruses …

Is there a “biological desert” with the discovery of new plant viruses? A retrospective analysis for new fruit tree viruses

W Hou, S Li, S Massart - Frontiers in Microbiology, 2020 - frontiersin.org
High throughput sequencing technologies accelerated the pace of discovery and
identification of new viral species. Nevertheless, biological characterization of a new virus is …

Application of biotechniques for in vitro virus and viroid elimination in pome fruit crops

JC Bettoni, MR Wang, JW Li, X Fan, G Fazio… - …, 2024 - Am Phytopath Society
Sustainable production of pome fruit crops is dependent upon having virus-free planting
materials. The production and distribution of plants derived from virus-and viroid-negative …