Use of BODIPY (493/503) to visualize intramuscular lipid droplets in skeletal muscle

EE Spangenburg, SJP Pratt… - BioMed Research …, 2011 - Wiley Online Library
Triglyceride storage is altered across various chronic health conditions necessitating various
techniques to visualize and quantify lipid droplets (LDs). Here, we describe the utilization of …

The effect of exercise and nutrition on intramuscular fat metabolism and insulin sensitivity

CS Shaw, J Clark… - Annual review of …, 2010 - annualreviews.org
Intramuscular triacylglycerol (IMTG) is both a dynamic fat-storage depot that can expand
during periods of elevated lipid availability and a fatty acid source that can be utilized during …

Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease

A Mehlem, CE Hagberg, L Muhl, U Eriksson… - Nature protocols, 2013 - nature.com
Excess lipid accumulation in peripheral tissues is a key feature of many metabolic diseases.
Therefore, techniques for imaging and quantifying lipids in various tissues are important for …

Insulin resistance in adolescents with type 2 diabetes is associated with impaired exercise capacity

KJ Nadeau, PS Zeitler, TA Bauer… - The Journal of …, 2009 - academic.oup.com
Context: The incidence of pediatric type 2 diabetes (T2D) is rising, with unclear effects on the
cardiovascular system. Cardiopulmonary fitness, a marker of morbidity and mortality, is …

[HTML][HTML] A new method for non-invasive estimation of human muscle fiber type composition

A Baguet, I Everaert, P Hespel, M Petrovic, E Achten… - PLoS …, 2011 - journals.plos.org
Background It has been established that excellence in sports with short and long exercise
duration requires a high proportion of fast-twitch (FT) or type-II fibers and slow-twitch (ST) or …

Network distribution of mitochondria and lipid droplets in human muscle fibres

CS Shaw, DA Jones, AJM Wagenmakers - Histochemistry and cell biology, 2008 - Springer
The objective of the present study was to develop a combination of fluorescent stains that
would allow visualisation of the network of mitochondria and lipid droplets (intramyocellular …

Intramuscular lipid utilization during exercise: a systematic review, meta-analysis, and meta-regression

JR Stokie, G Abbott, KF Howlett… - Journal of Applied …, 2023 - journals.physiology.org
Intramuscular lipid (IMCL) utilization during exercise was controversial as numerous studies
did not observe a decline in IMCL content post-exercise when assessed in muscle biopsies …

Acute exercise increases the contact between lipid droplets and mitochondria independently of obesity and type 2 diabetes

ME de Almeida, N Ørtenblad… - The Journal of …, 2023 - Wiley Online Library
Intramuscular lipid droplets (LDs) and mitochondria are essential organelles in cellular
communication and metabolism, supporting local energy demands during muscle …

IMCL area density, but not IMCL utilization, is higher in women during moderate-intensity endurance exercise, compared with men

MC Devries, SA Lowther, AW Glover… - American Journal …, 2007 - journals.physiology.org
Women use more fat during endurance exercise as evidenced by a lower respiratory
exchange ratio (RER). The contribution of intramyocellular lipid (IMCL) to lipid oxidation …

Muscle lipid droplets: cellular signaling to exercise physiology and beyond

JT Seibert, CP Najt, TD Heden, DG Mashek… - Trends in Endocrinology …, 2020 - cell.com
Conventionally viewed as energy storage depots, lipid droplets (LDs) play a central role in
muscle lipid metabolism and intracellular signaling, as recognized by recent advances in …