Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Spectral imaging is a technology that integrates conventional imaging and spectroscopy to
get both spatial and spectral information from an object. Although this technology was …

The success of fluorescence experiments requires attention to experimental details and an
understanding of the instrumentation. There are also many potential artifacts that can distort …

In fluorescence microscopy, the fluorescence emission can be characterised not only by
intensity and position, but also by lifetime, polarization and wavelength. Fluorescence …

Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial
distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems …

An emerging theme in cell biology is that cell surface receptors need to be considered as
part of supramolecular complexes of proteins and lipids facilitating specific receptor …

A novel approach to reduce the multiple light scattering contribution in planar laser images
of atomizing sprays is reported. This new technique, named Structured Laser Illumination …

Fluorescence lifetime measurements of long excited-state lifetime, oxygen-quenched
ruthenium dyes are emerging as methods for intracellular oxygen sensing. Fluorescence …

In the field of fluorescence microscopy, there is continued demand for dynamic technologies
that can exploit the complete information from every pixel of an image. One imaging …

Fluorescence imaging is used widely in microscopy and macroscopic imaging applications
for fields ranging from biomedicine to materials science. A critical component for any …