Pushing the frontiers: tools for monitoring neurotransmitters and neuromodulators
Neurotransmitters and neuromodulators have a wide range of key roles throughout the
nervous system. However, their dynamics in both health and disease have been challenging …
nervous system. However, their dynamics in both health and disease have been challenging …
Fluorescent proteins and genetically encoded biosensors
M Wang, Y Da, Y Tian - Chemical Society Reviews, 2023 - pubs.rsc.org
The genetically encoded fluorescent sensors convert chemical and physical signals into
light. They are powerful tools for the visualisation of physiological processes in living cells …
light. They are powerful tools for the visualisation of physiological processes in living cells …
Directed evolution of adeno-associated virus for efficient gene delivery to microglia
As the resident immune cells in the central nervous system (CNS), microglia orchestrate
immune responses and dynamically sculpt neural circuits in the CNS. Microglial dysfunction …
immune responses and dynamically sculpt neural circuits in the CNS. Microglial dysfunction …
Sensitive multicolor indicators for monitoring norepinephrine in vivo
Genetically encoded indicators engineered from G-protein-coupled receptors are important
tools that enable high-resolution in vivo neuromodulator imaging. Here, we introduce a …
tools that enable high-resolution in vivo neuromodulator imaging. Here, we introduce a …
[HTML][HTML] Real-time denoising enables high-sensitivity fluorescence time-lapse imaging beyond the shot-noise limit
A fundamental challenge in fluorescence microscopy is the photon shot noise arising from
the inevitable stochasticity of photon detection. Noise increases measurement uncertainty …
the inevitable stochasticity of photon detection. Noise increases measurement uncertainty …
A genetically encoded sensor measures temporal oxytocin release from different neuronal compartments
Oxytocin (OT), a peptide hormone and neuromodulator, is involved in diverse physiological
and pathophysiological processes in the central nervous system and the periphery …
and pathophysiological processes in the central nervous system and the periphery …
Fluorescence imaging of neural activity, neurochemical dynamics, and drug-specific receptor conformation with genetically encoded sensors
C Dong, Y Zheng, K Long-Iyer… - Annual review of …, 2022 - annualreviews.org
Recent advances in fluorescence imaging permit large-scale recording of neural activity and
dynamics of neurochemical release with unprecedented resolution in behaving animals …
dynamics of neurochemical release with unprecedented resolution in behaving animals …
Neuronal activity-induced, equilibrative nucleoside transporter-dependent, somatodendritic adenosine release revealed by a GRAB sensor
The purinergic signaling molecule adenosine (Ado) modulates many physiological and
pathological functions in the brain. However, the exact source of extracellular Ado remains …
pathological functions in the brain. However, the exact source of extracellular Ado remains …
[HTML][HTML] Adenosine-independent regulation of the sleep–wake cycle by astrocyte activity
Astrocytes play a crucial role in regulating sleep–wake behavior, and adenosine signaling is
generally thought to be involved. Here we show multiple lines of evidence supporting that …
generally thought to be involved. Here we show multiple lines of evidence supporting that …
[HTML][HTML] Lights, fiber, action! A primer on in vivo fiber photometry
Fiber photometry is a key technique for characterizing brain-behavior relationships in vivo.
Initially, it was primarily used to report calcium dynamics as a proxy for neural activity via …
Initially, it was primarily used to report calcium dynamics as a proxy for neural activity via …