According to Darwin's theory, endless evolution leads to a revolution. One such example is
the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)–Cas system, an …

As important genome editing tools, CRISPR/Cas systems, especially those based on type II
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …

Corynebacterium glutamicum is an important industrial metabolite producer that is difficult to
genetically engineer. Although the Streptococcus pyogenes (Sp) CRISPR-Cas9 system has …

Amino acid fermentation is one of the major pillars of industrial biotechnology. The multi-
billion USD amino acid market is rising steadily and is diversifying. Metabolic engineering is …

Corynebacterium glutamicum is a major workhorse in industrial biotechnology. For the past
several decades, this soil bacterium has been used to produce the amino acids l-glutamate …

In the period 1985 to 1995 applications of biocatalysis, driven by the need for more
sustainable manufacture of chemicals and catalytic,(enantio) selective methods for the …

Amino acids are attractive and promising biochemicals with market capacity requirements
constantly increasing. Their applicability ranges from animal feed additives, flavour …

The term 'clustered regularly interspaced short palindromic repeats'(CRISPR) has recently
become synonymous with the genome-editing revolution. The RNA-guided endonuclease …

Highlights•Microbial cell factories are becoming important in sustainable chemical
production.•Systems metabolic engineering allows efficient microbial cell factory …

Methods to regulate gene expression programs in bacterial cells are limited by the absence
of effective gene activators. To address this challenge, we have developed synthetic …