[HTML][HTML] Real-time polymerase chain reaction: Current techniques, applications, and role in COVID-19 diagnosis

IM Artika, YP Dewi, IM Nainggolan, JE Siregar… - Genes, 2022 - mdpi.com
Successful detection of the first SARS-CoV-2 cases using the real-time polymerase chain
reaction (real-time PCR) method reflects the power and usefulness of this technique. Real …

Comprehending COVID-19 diagnostic tests and greenness assessment of its reported detection methods

M El-Maghrabey, G Magdy, HM Hashem… - TrAC Trends in …, 2023 - Elsevier
Abstract In 2020, World Health Organization declared a pandemic due to acute respiratory
syndrome coronavirus (SARS‐CoV‐2). COVID-19 is an extremely infective disease that has …

[HTML][HTML] Epidemic and evolutionary characteristics of swine enteric viruses in South-Central China from 2018 to 2021

C Li, H Lu, C Geng, K Yang, W Liu, Z Liu, F Yuan… - Viruses, 2022 - mdpi.com
Swine enteric viruses are a major cause of piglet diarrhea, causing a devastating impact on
the pork industry. To further understand the molecular epidemiology and evolutionary …

Melatonin and other indoles show antiviral activities against swine coronaviruses in vitro at pharmacological concentrations

X Zhai, N Wang, H Jiao, J Zhang, C Li… - Journal of pineal …, 2021 - Wiley Online Library
The current coronavirus disease 2019 (COVID‐19) pandemic, caused by severe acute
respiratory syndrome coronavirus 2 (SARS‐CoV‐2), highlights major gaps in our knowledge …

[HTML][HTML] Reverse transcription–enzymatic recombinase amplification coupled with CRISPR-Cas12a for rapid detection and differentiation of PEDV wild-type strains …

K Yang, Y Liang, Y Li, Q Liu, W Zhang, D Yin… - Analytical and …, 2021 - Springer
Porcine epidemic diarrhea virus (PEDV) is an enteric coronavirus that causes acute watery
diarrhea and vomiting in unweaned piglets, and is associated with high mortality, thus …

[HTML][HTML] Development and validation of multiplex one-step qPCR/RT-qPCR assays for simultaneous detection of SARS-CoV-2 and pathogens associated with feline …

CJ Thieulent, M Carossino, L Peak, W Wolfson… - Plos one, 2024 - journals.plos.org
Feline respiratory disease complex (FRDC) is caused by a wide range of viral and bacterial
pathogens. Both Influenza A virus (IAV) and Severe Acute Respiratory Syndrome …

[HTML][HTML] Fast track diagnostic tools for clinical management of sepsis: Paradigm shift from conventional to advanced methods

E Gupta, J Saxena, S Kumar, U Sharma, S Rastogi… - Diagnostics, 2023 - mdpi.com
Sepsis is one of the deadliest disorders in the new century due to specific limitations in early
and differential diagnosis. Moreover, antimicrobial resistance (AMR) is becoming the …

[HTML][HTML] Development of a novel double antibody sandwich ELISA for quantitative detection of porcine deltacoronavirus antigen

W Wang, J Li, B Fan, X Zhang, R Guo, Y Zhao, J Zhou… - Viruses, 2021 - mdpi.com
Porcine deltacoronavirus (PDCoV) can cause diarrhea and dehydration in newborn piglets.
Here, we developed a double antibody sandwich quantitative enzyme-linked …

[HTML][HTML] Development and validation of a panel of one-step four-Plex qPCR/RT-qPCR assays for simultaneous detection of SARS-CoV-2 and other pathogens …

CJ Thieulent, M Carossino, L Peak, K Strother… - Viruses, 2023 - mdpi.com
Canine infectious respiratory disease complex (CIRDC) is the primary cause of respiratory
disease in the canine population and is caused by a wide array of viruses and bacterial …

[HTML][HTML] Development and clinical applications of a 5-Plex real-time RT-PCR for swine enteric coronaviruses

JH Zhu, G Rawal, E Aljets, W Yim-Im, YL Yang… - Viruses, 2022 - mdpi.com
A PEDV/PDCoV/TGEV/SADS-CoV/XIPC 5-plex real-time RT-PCR was developed and
validated for the simultaneous detection and differentiation of four swine enteric …