Technologies to profoundly engineer biology are becoming increasingly affordable,
powerful, and accessible to a widening group of actors. While offering tremendous potential …

Fungal pathogens pose a global threat to human health, with Candida albicans among the
leading killers. Systematic analysis of essential genes provides a powerful strategy to …

Genome-wide phenotypic screens in the budding yeast Saccharomyces cerevisiae, enabled
by its knockout collection, have produced the largest, richest, and most systematic …

An essential gene encodes for a cellular function that is required for viability. Although
viability is a straightforward phenotype to analyze in yeast, defining a gene as essential is …

Copy number variants (CNVs), duplications and deletions of genomic sequences, contribute
to evolutionary adaptation but can also confer deleterious effects and cause disease …

Systems that allow researchers to precisely control the expression of genes are fundamental
to biological research, biotechnology, and synthetic biology. However, few inducible gene …

High-throughput sequencing data enables the comprehensive study of genomes and the
variation therein. Essential for the interpretation of this genomic data is a thorough …

Chemical probes are important tools for understanding biological systems. However,
because of the huge combinatorial space of targets and potential compounds, traditional …

Overexpression can help life adapt to stressful environments, making an examination of
overexpressed genes valuable for understanding stress tolerance mechanisms. However, a …

A network of transcription factors (TFs) coordinates transcription with cell cycle events in
eukaryotes. Most TFs in the network are phosphorylated by cyclin-dependent kinase (CDK) …