CRISPR-based diagnostics
MM Kaminski, OO Abudayyeh, JS Gootenberg… - Nature Biomedical …, 2021 - nature.com
The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic
intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic …
intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic …
Diagnosing COVID-19: the disease and tools for detection
B Udugama, P Kadhiresan, HN Kozlowski… - ACS …, 2020 - ACS Publications
COVID-19 has spread globally since its discovery in Hubei province, China in December
2019. A combination of computed tomography imaging, whole genome sequencing, and …
2019. A combination of computed tomography imaging, whole genome sequencing, and …
Current and emerging trends in techniques for plant pathogen detection
M Venbrux, S Crauwels, H Rediers - Frontiers in Plant Science, 2023 - frontiersin.org
Plant pathogenic microorganisms cause substantial yield losses in several economically
important crops, resulting in economic and social adversity. The spread of such plant …
important crops, resulting in economic and social adversity. The spread of such plant …
Exploration of nanozymes in viral diagnosis and therapy
Nanozymes are nanomaterials with similar catalytic activities to natural enzymes. Compared
with natural enzymes, they have numerous advantages, including higher physiochemical …
with natural enzymes, they have numerous advantages, including higher physiochemical …
The ultimate qPCR experiment: producing publication quality, reproducible data the first time
SC Taylor, K Nadeau, M Abbasi, C Lachance… - Trends in …, 2019 - cell.com
Quantitative PCR (qPCR) is one of the most common techniques for quantification of nucleic
acid molecules in biological and environmental samples. Although the methodology is …
acid molecules in biological and environmental samples. Although the methodology is …
REASSURED multiplex diagnostics: a critical review and forecast
JA Otoo, TS Schlappi - Biosensors, 2022 - mdpi.com
The diagnosis of infectious diseases is ineffective when the diagnostic test does not meet
one or more of the necessary standards of affordability, accessibility, and accuracy. The …
one or more of the necessary standards of affordability, accessibility, and accuracy. The …
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …
Current trends in microsatellite genotyping
E Guichoux, L Lagache, S Wagner… - Molecular ecology …, 2011 - Wiley Online Library
Microsatellites have been popular molecular markers ever since their advent in the late
eighties. Despite growing competition from new genotyping and sequencing techniques, the …
eighties. Despite growing competition from new genotyping and sequencing techniques, the …
COVID-19 diagnosis: a comprehensive review of the RT-qPCR method for detection of SARS-CoV-2
The world is grappling with the coronavirus disease 2019 (COVID-19) pandemic, the
causative agent of which is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) …
causative agent of which is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) …
PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings
S Yang, RE Rothman - The Lancet infectious diseases, 2004 - thelancet.com
Molecular diagnostics are revolutionising the clinical practice of infectious disease. Their
effects will be significant in acute-care settings where timely and accurate diagnostic tools …
effects will be significant in acute-care settings where timely and accurate diagnostic tools …