Programmable gene-editing tools have transformed the life sciences and have shown
potential for the treatment of genetic disease. Among the CRISPR–Cas technologies that …

Precise replacement of an allele with an elite allele controlling an important agronomic trait
in a predefined manner by gene editing technologies is highly desirable in crop …

Using genetic resistance against bacterial blight (BB) caused by Xanthomonas oryzae
pathovar oryzae (Xoo) is a major objective in rice breeding programmes. Prime editing (PE) …

Prime editing is limited by low efficiency in plants. Here, we develop an upgraded
engineered plant prime editor in hexaploid wheat, ePPEplus, by introducing a V223A …

Cereal crops, including triticeae species (barley, wheat, rye), as well as edible cereals
(wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption …

Prime editing (PE) is a precise genome manipulation technology based on the “search and
replace” approach of the CRISPR-Cas9 system, while it does not require the exogenous …

Crop improvement by genome editing involves the targeted alteration of genes to improve
plant traits, such as stress tolerance, disease resistance or nutritional content. Techniques …

Low efficiency is the main obstacle to using prime editing in maize (Zea mays). Recently,
prime‐editing efficiency was greatly improved in mammalian cells and rice (Oryza sativa) …

Increased understanding of plant genetics and the development of powerful and easier-to-
use gene editing tools over the past century have revolutionized humankind's ability to …

Genetic modifications are made through diverse mutagenesis techniques for crop
improvement programs. Among these mutagenesis tools, the traditional methods involve …