Delivery of CRISPR-Cas tools for in vivo genome editing therapy: Trends and challenges
EA Taha, J Lee, A Hotta - Journal of Controlled Release, 2022 - Elsevier
The discovery of clustered regularly interspaced short palindromic repeats (CRISPR)
genome editing technology opened the door to provide a versatile approach for treating …
genome editing technology opened the door to provide a versatile approach for treating …
CRISPR-based genome editing through the lens of DNA repair
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity
Cytosine base editors (CBEs) are larger and can suffer from higher off-target activity or lower
on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that …
on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that …
Re-engineering the adenine deaminase TadA-8e for efficient and specific CRISPR-based cytosine base editing
Cytosine base editors (CBEs) efficiently generate precise C· G-to-T· A base conversions, but
the activation-induced cytidine deaminase/apolipoprotein B mRNA-editing enzyme catalytic …
the activation-induced cytidine deaminase/apolipoprotein B mRNA-editing enzyme catalytic …
Engineering a precise adenine base editor with minimal bystander editing
Adenine base editors (ABEs) catalyze A-to-G transitions showing broad applications, but
their bystander mutations and off-target editing effects raise safety concerns. Through …
their bystander mutations and off-target editing effects raise safety concerns. Through …
Improved cytosine base editors generated from TadA variants
Cytosine base editors (CBEs) enable programmable genomic C· G-to-T· A transition
mutations and typically comprise a modified CRISPR–Cas enzyme, a naturally occurring …
mutations and typically comprise a modified CRISPR–Cas enzyme, a naturally occurring …
In vivo somatic cell base editing and prime editing
Recent advances in genome editing technologies have magnified the prospect of single-
dose cures for many genetic diseases. For most genetic disorders, precise DNA correction is …
dose cures for many genetic diseases. For most genetic disorders, precise DNA correction is …
High-purity production and precise editing of DNA base editing ribonucleoproteins
Ribonucleoprotein (RNP) complex–mediated base editing is expected to be greatly
beneficial because of its reduced off-target effects compared to plasmid-or viral vector …
beneficial because of its reduced off-target effects compared to plasmid-or viral vector …
Base editing of organellar DNA with programmable deaminases
Mitochondria and chloroplasts are organelles that include their own genomes, which
encode key genes for ATP production and carbon dioxide fixation, respectively. Mutations in …
encode key genes for ATP production and carbon dioxide fixation, respectively. Mutations in …
In the business of base editors: evolution from bench to bedside
EM Porto, AC Komor - PLoS biology, 2023 - journals.plos.org
With the advent of recombinant DNA technology in the 1970s, the idea of using gene
therapies to treat human genetic diseases captured the interest and imagination of scientists …
therapies to treat human genetic diseases captured the interest and imagination of scientists …