Enabling technology and core theory of synthetic biology

XE Zhang, C Liu, J Dai, Y Yuan, C Gao, Y Feng… - Science China Life …, 2023 - Springer
Synthetic biology provides a new paradigm for life science research (“build to learn”) and
opens the future journey of biotechnology (“build to use”). Here, we discuss advances of …

Design of artificial enzymes bearing several active centers: New trends, opportunities and problems

D Carballares, R Morellon-Sterling… - International Journal of …, 2022 - mdpi.com
Harnessing enzymes which possess several catalytic activities is a topic where intense
research has been carried out, mainly coupled with the development of cascade reactions …

A novel, genetically encoded whole-cell biosensor for directed evolution of myrcene synthase in Escherichia coli

C Chen, J Liu, G Yao, S Bao, X Wan, F Wang… - Biosensors and …, 2023 - Elsevier
Abstract β-myrcene is a high-value acyclic monoterpene. The low activity of myrcene
synthase resulted to low biosynthetic titer of it. Biosensor is a promising tool applied for …

[HTML][HTML] Advances in metabolic engineering of yeasts for the production of fatty acid-derived hydrocarbon fuels

R Lu, TQ Shi, L Lin, R Ledesma-Amaro, XJ Ji… - Green Chemical …, 2022 - Elsevier
The reliance of the transport sector on fossil fuels has led to increasing concerns related to
sustainability and environmental impact. Growing petroleum shortages and global climate …

[HTML][HTML] Enzyme immobilization studied through molecular dynamic simulations

N Bhattacharjee, L Alonso-Cotchico… - … in Bioengineering and …, 2023 - frontiersin.org
In recent years, simulations have been used to great advantage to understand the structural
and dynamic aspects of distinct enzyme immobilization strategies, as experimental …

[HTML][HTML] Data-driven strategies for the computational design of enzyme thermal stability: trends, perspectives, and prospects: Data-driven strategies for enzyme …

Z Dou, Y Sun, X Jiang, X Wu, Y Li, B Gong… - Acta Biochimica et …, 2023 - ncbi.nlm.nih.gov
Thermal stability is one of the most important properties of enzymes, which sustains life and
determines the potential for the industrial application of biocatalysts. Although traditional …

Progress and Prospects of Natural Glycoside Sweetener Biosynthesis: A Review

G Qu, Y Liu, Q Ma, J Li, G Du, L Liu… - Journal of Agricultural and …, 2023 - ACS Publications
To achieve an adequate sense of sweetness with a healthy low-sugar diet, it is necessary to
explore and produce sugar alternatives. Recently, glycoside sweeteners and their …

In Vitro Biotransformation of High-Titer d-Glucarate by Stepwise-Added Enzyme Cocktails

Y Li, C You, YHPJ Zhang - Organic Process Research & …, 2024 - ACS Publications
d-Glucaric acid (GA), one of the polyhydroxy dicarboxylic acids, is known for its great
potentials in the polymer, food, detergent, and pharmaceutical industries. Here, we …

Impact of single-residue mutations on protein thermal stability: The case of threonine 83 of BC2L-CN lectin

M Hoya, R Matsunaga, S Nagatoishi, T Ide… - International Journal of …, 2024 - Elsevier
The thermal stability of trimeric lectin BC2L-CN was investigated and found to be
considerably altered when mutating residue 83, originally a threonine, located at the fucose …

[HTML][HTML] 赖氨酸脱羧酶分子改造及固定化合成1, 5-戊二胺研究进展

刘思敏, 齐海山 - 生物工程学报, 2022 - cjb.ijournals.cn
1, 5-戊二胺又名尸胺, 是一种重要的生物基聚酰胺生产原料, 可以与二元羧酸缩合生成生物基聚
酰胺PA5X, 其性能可以与石油基聚酰胺材料媲美. 生物基聚酰胺以可再生能源为底物, 如淀粉 …