The low carbon yield from native metabolic machinery produces unfavorable process
economics during the biological conversion of substrates to desirable bioproducts. To obtain …

Aspartate kinase (AK) is a key enzyme involved in catalyzing the first step of the aspartate-
derived amino acid biosynthesis, including L-lysine and L-threonine, which is regulated by …

Background Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) containing various
chain length monomers from C6 to C14 have more applications besides sustainable and …

The strategic design of this study aims at fermentative succinate production with high
volumetric productivity in engineered Escherichia coli. An E. coli YL106/pSCsfcA was …

The biosynthesis of polyhydroxyalkanoate copolymers in Escherichia coli from unrelated
carbon sources becomes attractive nowadays. We previously developed a poly …

Shikimate is a key intermediate in the synthesis of neuraminidase inhibitors. Compared with
traditional methods, microbial production of shikimate has the advantages of environmental …

Abstract l-Serine is a nonessential amino acid, but plays a crucial role as a building block for
cell growth. Currently, l-serine production is mainly dependent on enzymatic or cellular …

Zero-valent sulfur is accumulated in the cytoplasm of certain sulfur-oxidizing or reducing
microorganisms. When these microorganisms are unable to metabolize zero-valent sulfur …

The recombination of short homologous ends in Escherichia coli has been known for 30
years, and it is often used for both site‐directed mutagenesis and in vivo cloning. For …

Acetate is a potential low-cost carbon source that can be generated by biological and
chemical processes. In this study, deletion of sdhAB encoding succinate dehydrogenase …