A series of spectacular scientific discoveries and technological advances in the second half
of the 20th century have provided the basis for the ongoing genome editing revolution. The …

Highlights•Efficient microbial cell factories are developed by systems metabolic
engineering.•Recent advances in systems metabolic engineering tools and strategies are …

Filamentous fungi are competitive hosts for the production of drugs, proteins, and chemicals.
However, their utility is limited by screening methods and low throughput. In this work, a …

Solubility and folding stability are key concerns for difficult-to-express proteins (DEPs)
restricted by amino acid sequences and superarchitecture, resolved by the precise …

Cofactor imbalance obstructs the productivities of metabolically engineered cells. Herein, we
employed a minimally perturbing system, xylose reductase and lactose (XR/lactose), to …

Plant breeding has been closely aligned with the development of civilisations and continues
to be important for the supply of nutritious food and a key factor in reducing poverty and …

l-Histidine is an essential proteinogenic amino acid in food with extensive applications in the
pharmaceutical field. Herein, we constructed a Corynebacterium glutamicum recombinant …

The success of forward metabolic engineering depends on a thorough understanding of the
behaviour of a heterologous metabolic pathway within its host. We have recently described …

Parageobacillus thermoglucosidasius is a thermophilic and facultatively anaerobic microbe,
which is emerging as one of the most promising thermophilic model organisms for metabolic …

Gastrodin, 4-hydroxybenzyl alcohol-4-O-β-D-glucopyranoside, has been widely used in the
treatment of neurogenic and cardiovascular diseases. Currently, gastrodin biosynthesis is …