As important genome editing tools, CRISPR/Cas systems, especially those based on type II
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …

Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …

The RNA-guided CRISPR-associated (Cas) nucleases are versatile tools for genome editing
in various organisms. The large sizes of the commonly used Cas9 and Cas12a nucleases …

The increasing emergence and dissemination of multidrug resistant (MDR) bacterial
pathogens accelerate the desires for new antibiotics. Natural products dominate the …

Type V CRISPR-associated systems (Cas) 12 family nucleases are considered to have
evolved from transposon-associated TnpB, and several of these nucleases have been …

Polysaccharide capsule is the main virulence factor of K. pneumoniae, a major pathogen of
bloodstream infections in humans. While more than 80 capsular serotypes have been …

Gut colonization with multidrug-resistant (MDR) bacteria enhances the risk of bloodstream
infections in susceptible individuals. We demonstrate highly variable degrees of ex vivo …

Studies of the gut microbiota have revealed associations between specific bacterial species
or community compositions with health and disease, yet the causal mechanisms underlying …

Cefiderocol has been approved in the United States and Europe but not in China. We aim to
evaluate carbapenem-resistant Enterobacterales (CRE) susceptibility to cefiderocol to …

Gene therapy is limited by inefficient delivery of large clustered regularly interspaced short
palindromic repeat (CRISPR) effectors, such as Cas9 and Cas12a nucleases. Cas12f …