Diagnosis of invasive fungal infections: challenges and recent developments

W Fang, J Wu, M Cheng, X Zhu, M Du, C Chen… - Journal of biomedical …, 2023 - Springer
Background The global burden of invasive fungal infections (IFIs) has shown an upsurge in
recent years due to the higher load of immunocompromised patients suffering from various …

Fungal diversity revisited: 2.2 to 3.8 million species

DL Hawksworth, R Lücking - Microbiology spectrum, 2017 - Am Soc Microbiol
The question of how many species of Fungi there are has occasioned much speculation,
with figures mostly posited from around half a million to 10 million, and in one extreme case …

Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?

R Lücking, MC Aime, B Robbertse, AN Miller… - IMA fungus, 2020 - Springer
True fungi (Fungi) and fungus-like organisms (eg Mycetozoa, Oomycota) constitute the
second largest group of organisms based on global richness estimates, with around 3 …

A few Ascomycota taxa dominate soil fungal communities worldwide

E Egidi, M Delgado-Baquerizo, JM Plett, J Wang… - Nature …, 2019 - nature.com
Despite having key functions in terrestrial ecosystems, information on the dominant soil fungi
and their ecological preferences at the global scale is lacking. To fill this knowledge gap, we …

Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher …

D Vu, M Groenewald, M De Vries… - Studies in …, 2019 - ingentaconnect.com
Species identification lies at the heart of biodiversity studies that has in recent years
favoured DNA-based approaches. Microbial Biological Resource Centres are a rich source …

Fungal identification using molecular tools: a primer for the natural products research community

HA Raja, AN Miller, CJ Pearce… - Journal of natural …, 2017 - ACS Publications
Fungi are morphologically, ecologically, metabolically, and phylogenetically diverse. They
are known to produce numerous bioactive molecules, which makes them very useful for …

Assessment of BOLD and GenBank–Their accuracy and reliability for the identification of biological materials

KA Meiklejohn, N Damaso, JM Robertson - PloS one, 2019 - journals.plos.org
Taxonomic identification of biological materials can be achieved through DNA barcoding,
where an unknown “barcode” sequence is compared to a reference database. In many …

Tag jumps illuminated–reducing sequence‐to‐sample misidentifications in metabarcoding studies

IB Schnell, K Bohmann… - Molecular ecology …, 2015 - Wiley Online Library
Metabarcoding of environmental samples on second‐generation sequencing platforms has
rapidly become a valuable tool for ecological studies. A fundamental assumption of this …

[PDF][PDF] Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi

L Tedersoo, S Anslan, M Bahram, S Põlme, T Riit… - …, 2015 - mycokeys.pensoft.net
Rapid development of high-throughput (HTS) molecular identification methods has
revolutionized our knowledge about taxonomic diversity and ecology of fungi. However …

PacBio metabarcoding of Fungi and other eukaryotes: errors, biases and perspectives

L Tedersoo, A Tooming‐Klunderud… - New Phytologist, 2018 - Wiley Online Library
Second‐generation, high‐throughput sequencing methods have greatly improved our
understanding of the ecology of soil microorganisms, yet the short barcodes (< 500 bp) …