The integration of a DNA copy of the viral RNA genome into host chromatin is the defining
step of retroviral replication. This enzymatic process is catalyzed by the virus-encoded …

Although many aspects of enzyme catalysis have been constructively analyzed, 1-52 there
are still many aspects that are imperfectly understood. Of particular interest in this regard are …

Insolubility of full-length HIV-1 integrase (IN) limited previous structure analyses to individual
domains. By introducing five point mutations, we engineered a more soluble IN that allowed …

Integrase (IN), an essential enzyme of human immunodeficiency virus (HIV), is an attractive
antiretroviral drug target. The antiviral activity and resistance profile in vitro of a novel IN …

HIV-1 integrase is a multidomain enzyme which is required for the integration of viral DNA
into the host genome. It is one of three enzymes of HIV, the others being the Reverse …

Breakthroughs in enzyme immobilization have enabled increased enzyme recovery and
reusability, leading to significant decreases in the cost of enzyme use and fueling …

Antiretroviral therapy has led to a significant decrease in human immunodeficiency virus
(HIV)-related mortality. Approved antiretroviral drugs target different steps of the viral life …

Discovery of diketoacid-containing compounds as HIV-1 integrase (IN) inhibitors played a
major role in validating this enzyme as an important target for the development of …

Trypsin and chymotrypsin are both serine proteases with high sequence and structural
similarities, but with different substrate specificity. Previous experiments have demonstrated …

Integration of viral DNA into the host chromosome is an essential step in the life cycle of
retroviruses and is facilitated by the viral integrase enzyme. The first generation of integrase …