Antimicrobial peptides are of great interest due to their potential application as novel
antibiotics. Large quantities of highly purified peptides are required to meet the needs of …

Human defensins are a family of cationic antimicrobial peptides with molecular weights of 4–
5kDa, containing a conserved six disulphide-linked cysteine motif. During the last two …

Background Human defensin peptides with broad‐spectrum antimicrobial activity have been
implicated in the human defence response towards microbial invasion. Two families of …

Abstract β‐defensins are a large family of multiple disulfide‐bonded peptides occurring in
mammals and birds. They play an important role in the innate immune system, directly killing …

Biomanufacturing requires the stable production of active biological molecules including
proteins. However, a significant percentage of genes cannot be expressed efficiently in a …

Defensins are small, β-sheet-rich, cationic peptides found in many organisms. All defensins
have amphiphilic properties, which are central for antimicrobial activities of the proteins. The …

Recombinant production of non-native peptides requires using protein fusion technology to
prevent peptide degradation by host-cell proteases. In this work, we have used SUMO …

Abstract Human beta-defensin-4 (hBD4) is a cationic 50-amino acid antimicrobial peptide
with three conserved cysteine disulfide bonds. It exhibits a broad antimicrobial spectrum …

Human beta-defensin-2 (hBD2), a small cationic peptide, exhibits a broad range of
antimicrobial activity and does not cause microbial resistance. In order to produce hBD2 …

Recombinant protein production in Escherichia coli often results in a dramatic cellular stress
response best characterized by a decrease in overall cell fitness. We determined that the …