[HTML][HTML] NMR of paramagnetic metalloproteins in solution: Ubi venire, quo vadis?

IB Trindade, A Coelho, F Cantini, M Piccioli… - Journal of Inorganic …, 2022 - Elsevier
Metalloproteins represent a substantial fraction of the proteome where they have an
outsized contribution to enzymology. This stems from the reactivity of transition metals found …

[HTML][HTML] Extracytoplasmic function sigma factors governing production of the primary siderophores in pathogenic Burkholderia species

A Grove - Frontiers in microbiology, 2022 - frontiersin.org
Bacteria respond to changing environments by modulating their gene expression programs.
One of the mechanisms by which this may be accomplished is by substituting the primary σ …

Relaxation-based NMR assignment: Spotlights on ligand binding sites in human CISD3

D Grifagni, JM Silva, F Cantini, M Piccioli… - Journal of Inorganic …, 2023 - Elsevier
CISD3 is a mitochondrial protein belonging to the NEET proteins family, bearing two [Fe 2 S
2] clusters coordinated by CDGSH domains. At variance with the other proteins of the NEET …

Paramagnetic NMR to study iron sulfur proteins: 13C detected experiments illuminate the vicinity of the metal center

L Querci, D Grifagni, IB Trindade, JM Silva… - Journal of Biomolecular …, 2023 - Springer
The robustness of NMR coherence transfer in proximity of a paramagnetic center depends
on the relaxation properties of the nuclei involved. In the case of Iron-Sulfur Proteins …

Production and Potential Genetic Pathways of Three Different Siderophore Types in Streptomyces tricolor Strain HM10

M Rehan, H Barakat, IS Almami, KA Qureshi… - Fermentation, 2022 - mdpi.com
Siderophores are iron-chelating low-molecular-weight compounds that bind iron (Fe3+) with
a high affinity for transport into the cell. The newly isolated strain Streptomyces tricolor HM10 …

NMR of Paramagnetic Proteins: 13C Derived Paramagnetic Relaxation Enhancements Are an Additional Source of Structural Information in Solution

L Querci, IB Trindade, M Invernici, JM Silva, F Cantini… - Magnetochemistry, 2023 - mdpi.com
In paramagnetic metalloproteins, longitudinal relaxation rates of 13C′ and 13Cα nuclei can
be measured using 13C detected experiments and converted into electron spin-nuclear spin …

1H, 13C and 15N assignment of the human mitochondrial paramagnetic iron–sulfur protein CISD3

JM Silva, D Grifagni, F Cantini, M Piccioli - Biomolecular NMR …, 2023 - Springer
CISD3 is a mitochondrial protein that contains two [2Fe–2S] clusters. This protein is
overexpressed in some types of cancer, so it has emerged as a potential drug target. A …

The long-standing relationship between Paramagnetic NMR and Iron-Sulfur proteins: the mitoNEET example. An old method for new stories or the other way around?

F Camponeschi, A Gallo, M Piccioli… - Magnetic Resonance …, 2021 - mr.copernicus.org
Paramagnetic NMR spectroscopy and iron-sulfur (Fe–S) proteins have maintained a
synergic relationship for decades. Indeed, the hyperfine shifts with their temperature …

The role of integration host factor in biofilm and virulence of high-alcohol-producing Klebsiella pneumoniae

Z Fan, T Fu, Z Li, B Du, X Cui, R Zhang… - Microbiology …, 2023 - Am Soc Microbiol
Klebsiella pneumoniae is a well-known human nosocomial pathogen with an arsenal of
virulence factors, including capsular polysaccharides (CPS), fimbriae, flagella, and …

SifR is an Rrf2-family quinone sensor associated with catechol iron uptake in Streptococcus pneumoniae D39

Y Zhang, JE Martin, KA Edmonds, ME Winkler… - Journal of Biological …, 2022 - ASBMB
Streptococcus pneumoniae (pneumococcus) is a Gram-positive commensal and human
respiratory pathogen. How this bacterium satisfies its nutritional iron (Fe) requirement in the …