Metagenomics approaches for the detection and surveillance of emerging and recurrent plant pathogens

E Piombo, A Abdelfattah, S Droby, M Wisniewski… - Microorganisms, 2021 - mdpi.com
Globalization has a dramatic effect on the trade and movement of seeds, fruits and
vegetables, with a corresponding increase in economic losses caused by the introduction of …

Monitoring airborne inoculum for improved plant disease management. A review

H Van der Heyden, P Dutilleul, JB Charron… - Agronomy for …, 2021 - Springer
Global demand for pesticide-free food products is increasing rapidly. Crops of all types are,
however, under constant threat from various plant pathogens. To achieve adequate control …

Facilitating the adoption of high‐throughput sequencing technologies as a plant pest diagnostic test in laboratories: A step‐by‐step description

B Lebas, I Adams, M Al Rwahnih, S Baeyen… - EPPO …, 2022 - Wiley Online Library
High‐throughput sequencing (HTS) is a powerful tool that enables the simultaneous
detection and potential identification of any organisms present in a sample. The growing …

Guidelines for the reliable use of high throughput sequencing technologies to detect plant pathogens and pests

S Massart, I Adams, M Al Rwahnih… - Peer Community …, 2022 - peercommunityjournal.org
High-throughput sequencing (HTS) technologies have the potential to become one of the
most significant advances in molecular diagnostics. Their use by researchers to detect and …

Environmental DNA assessment of airborne plant and fungal seasonal diversity

E Banchi, CG Ametrano, E Tordoni, D Stanković… - Science of the Total …, 2020 - Elsevier
Environmental DNA (eDNA) metabarcoding and metagenomics analyses can improve
taxonomic resolution in biodiversity studies. Only recently, these techniques have been …

New Reports of Phytophthora Species in Plant Nurseries in Spain

B Mora-Sala, M León, A Pérez-Sierra, P Abad-Campos - Pathogens, 2022 - mdpi.com
The plant nursery industry has become an ideal reservoir for Phytophthora species and
other soilborne pathogens. In this context, isolation from tissues and soil of ornamental and …

Cryptic risks to forest biosecurity associated with the global movement of commercial seed

M Cleary, F Oskay, HT Doğmuş, A Lehtijärvi… - Forests, 2019 - mdpi.com
The import and export of tree seed carries with it risks of inadvertent introduction of pests
and pathogens to hitherto unaffected regions. Although trade in seed of specified trees is …

Comparison of qPCR and metabarcoding methods as tools for the detection of airborne inoculum of forest fungal pathogens

A Chandelier, J Hulin, G San Martin… - …, 2021 - Am Phytopath Society
Forest diseases caused by invasive fungal pathogens are becoming more common,
sometimes with dramatic consequences to forest ecosystems. The development of early …

High‐resolution biomonitoring of plant pathogens and plant species using metabarcoding of pollen pellet contents collected from a honey bee hive

ÉD Tremblay, MO Duceppe, GB Thurston… - Environmental …, 2019 - Wiley Online Library
The Canadian beekeeping industry is spread across the country, with the greatest
proportion of managed honey bee colonies occurring in the Prairie Provinces. Nationally …

Calonectria in the age of genes and genomes: Towards understanding an important but relatively unknown group of pathogens

JQ Li, MJ Wingfield, I Barnes… - Molecular Plant …, 2022 - Wiley Online Library
The genus Calonectria includes many aggressive plant pathogens causing diseases on
various agricultural crops as well as forestry and ornamental tree species. Some species …