Heparin has been recognized as a valuable anticoagulant and antithrombotic for several
decades and is still widely used in clinical practice for a variety of indications. The …

Glycosaminoglycans (GAGs) are important complex carbohydrates that participate in many
biological processes through the regulation of their various protein partners. Biochemical …

Basic fibroblast growth factor (bFGF) binds to heparan sulfate proteoglycans at the cell
surface and to receptors with tyrosine kinase activity. Prevention of binding between cell …

Perlecan is a modular heparan sulfate proteoglycan that is localized to cell surfaces and
within basement membranes. Its ability to interact with basic fibroblast growth factor (bFGF) …

Chitosans are versatile biopolymers with multiple biological activities and potential
applications. They are linear copolymers of glucosamine and N-acetylglucosamine defined …

Two techniques for determining enzyme kinetic constants using isothermal titration
microcalorimetry are presented. The methods are based on the proportionality between the …

The structural properties of fibroblast heparan sulfate (HS) that are necessary for it to bind
strongly to basic fibroblast growth factor (bFGF) have been investigated using bFGF affinity …

Glycosarninoglycans (GAGs) play an intricate role in the extracellular matrix (ECM), not only
as soluble components and polyelectrolytes, but also by specific interactions with growth …

Experiments based on interaction in free solution between basic fibroblast growth factor
(FGF-2) and saccharides related to heparin/heparan sulfate showed that the growth factor …

The activity of fibroblast growth factor 2 (FGF-2) is stringently controlled. Inactive in
undisturbed tissues, it is activated during injury and is critical for tissue repair. We find that …