Assays for noninvasive imaging of reporter gene expression

SS Gambhir, JR Barrio, HR Herschman… - Nuclear medicine and …, 1999 - Elsevier
Repeated, noninvasive imaging of reporter gene expression is emerging as a valuable tool
for monitoring the expression of genes in animals and humans. Monitoring of organ/cell …

Monitoring gene therapy with reporter gene imaging

P Ray, E Bauer, M Lyer, JR Barrio… - Seminars in nuclear …, 2001 - Elsevier
Rapid advances in imaging technologies and gene transfer strategies offer a great
opportunity to optimize clinical trials of human gene therapy. Reporter genes are emerging …

Imaging adenoviral-directed reporter gene expression in living animals with positron emission tomography

SS Gambhir, JR Barrio, ME Phelps… - Proceedings of the …, 1999 - National Acad Sciences
We are developing quantitative assays to repeatedly and noninvasively image expression of
reporter genes in living animals, using positron emission tomography (PET). We synthesized …

Comparison of radiolabeled nucleoside probes (FIAU, FHBG, and FHPG) for PET imaging of HSV1-tk gene expression

JG Tjuvajev, M Doubrovin, T Akhurst… - Journal of Nuclear …, 2002 - Soc Nuclear Med
The efficacy of 3 radiolabeled probes of current interest for imaging herpes simplex virus
type 1 thymidine kinase (HSV1-tk) expression in vivo with PET, including 124I-or 131I …

PET imaging of transgene expression

DC MacLaren, T Toyokuni, SR Cherry, JR Barrio… - Biological …, 2000 - Elsevier
A vital step in transgenic animal study and gene therapy is the ability to assay the extent of
transgene expression. Unfortunately, classic methods of assaying transgene expression …

Imaging gene expression: principles and assays

SS Gambhir, JR Barrio, HR Herschman… - Journal of Nuclear …, 1999 - Elsevier
Nuclear imaging has a rich history rooted in the development of tracers and imaging assays
for, the improved diagnosis and management of many different diseases. The last decade …

Synthesis of 8-[18F] fluoroguanine derivatives: in vivo probes for imaging gene expression with positron emission tomography

M Namavari, JR Barrio, T Toyokuni, SS Gambhir… - Nuclear medicine and …, 2000 - Elsevier
A new method for the preparation of 8-[18F] fluoroguanine derivatives based on a direct
radiofluorination reaction has been developed. The radiofluorination of ganciclovir (1a) with …

5-Alkynyl Analogs of Arabinouridine and 2 '-Deoxyuridine: Cytostatic Activity against Herpes Simplex Virus and Varicella-Zoster Thymidine Kinase Gene-Transfected …

WA Cristofoli, LI Wiebe, E De Clercq… - Journal of medicinal …, 2007 - ACS Publications
A group of arabinouridines (TMSEAU, EAU, IEAU-TA) and 2 '-deoxyuridines (TMSEDU,
EDU, IEDU) having a variety of substituents at the uracil C-5 position (trimethylsilylethynyl …

Feasibility of sodium/iodide symporter gene as a new imaging reporter gene: comparison with HSV1-tk

JH Shin, JK Chung, JH Kang, YJ Lee, KI Kim… - European journal of …, 2004 - Springer
Positron emission tomography (PET) imaging reporter genes, such as HSV1-tk and D 2
receptor genes, make it possible to visualise gene expression non-invasively and …

Hydrogermylation of 5-ethynyluracil nucleosides: formation of 5-(2-germylvinyl) uracil and 5-(2-germylacetyl) uracil nucleosides

Y Liang, JP Pitteloud, SF Wnuk - The Journal of organic chemistry, 2013 - ACS Publications
A stereoselective radical-mediated hydrogermylation of the protected 5-ethynyluracil
nucleosides with trialkyl-, triaryl,-or tris (trimethylsilyl) germanes gave (Z)-5-(2-germylvinyl) …