Spatial transcriptomics drives a new era in plant research

R Yin, K Xia, X Xu - The Plant Journal, 2023 - Wiley Online Library
Spatial transcriptomics drives a new era in plant research - Yin - 2023 - The Plant Journal - Wiley
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Variation in transcriptome size: are we getting the message?

JE Coate, JJ Doyle - Chromosoma, 2015 - Springer
The number of RNA molecules per cell (transcriptome size) is highly variable, differing
among and within cell types depending on cell size, stage of the cell cycle, ploidy level, age …

[HTML][HTML] Reference genes for accurate transcript normalization in citrus genotypes under different experimental conditions

V Mafra, KS Kubo, M Alves-Ferreira, M Ribeiro-Alves… - PloS one, 2012 - journals.plos.org
Real-time reverse transcription PCR (RT-qPCR) has emerged as an accurate and widely
used technique for expression profiling of selected genes. However, obtaining reliable …

Validation of reference genes for RT-qPCR studies of gene expression in banana fruit under different experimental conditions

L Chen, H Zhong, J Kuang, J Li, W Lu, J Chen - Planta, 2011 - Springer
Reverse transcription quantitative real-time PCR (RT-qPCR) is a sensitive technique for
quantifying gene expression, but its success depends on the stability of the reference gene …

[HTML][HTML] Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR

R Hu, C Fan, H Li, Q Zhang, YF Fu - BMC molecular biology, 2009 - Springer
Background Real-time quantitative reverse transcription PCR (RT-qPCR) data needs to be
normalized for its proper interpretation. Housekeeping genes are routinely employed for this …

An integrated transcriptome atlas of the crop model Glycine max, and its use in comparative analyses in plants

M Libault, A Farmer, T Joshi, K Takahashi… - The Plant …, 2010 - Wiley Online Library
Soybean (Glycine max L.) is a major crop providing an important source of protein and oil,
which can also be converted into biodiesel. A major milestone in soybean research was the …

An optimized protocol for stepwise optimization of real-time RT-PCR analysis

F Zhao, NA Maren, PZ Kosentka, YY Liao… - Horticulture …, 2021 - academic.oup.com
Computational tool-assisted primer design for real-time reverse transcription (RT) PCR
(qPCR) analysis largely ignores the sequence similarities between sequences of …

Evaluation of candidate reference genes for qPCR in maize

A Manoli, A Sturaro, S Trevisan, S Quaggiotti… - Journal of plant …, 2012 - Elsevier
Quantitative real-time PCR (qPCR) is a powerful tool to measure gene expression levels.
Accurate and reproducible results are dependent on the correct choice of the reference …

Complete Transcriptome of the Soybean Root Hair Cell, a Single-Cell Model, and Its Alteration in Response to Bradyrhizobium japonicum Infection

M Libault, A Farmer, L Brechenmacher… - Plant …, 2010 - academic.oup.com
Nodulation is the result of a mutualistic interaction between legumes and symbiotic soil
bacteria (eg soybean [Glycine max] and Bradyrhizobium japonicum) initiated by the infection …

植物实时荧光定量PCR 内参基因的特点及选择

袁伟, 万红建, 杨悦俭 - 植物学报, 2012 - chinbullbotany.com
实时荧光定量PCR (qRT-PCR) 具有灵敏度高, 特异性强, 重复的动态定量范围和高通量等优点,
是进行植物基因表达和转录分析最常用的技术手段之一. 选择合适的内参基因是正确运用实时 …