Ultrasensitive and highly specific lateral flow assays for point-of-care diagnosis
Lateral flow assays (LFAs) are paper-based point-of-care (POC) diagnostic tools that are
widely used because of their low cost, ease of use, and rapid format. Unfortunately …
widely used because of their low cost, ease of use, and rapid format. Unfortunately …
Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities
JE van Dongen, JTW Berendsen… - Biosensors and …, 2020 - Elsevier
With the trend of moving molecular tests from clinical laboratories to on-site testing, there is a
need for nucleic acid based diagnostic tools combining the sensitivity, specificity and …
need for nucleic acid based diagnostic tools combining the sensitivity, specificity and …
[HTML][HTML] Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay
The recent outbreak of novel coronavirus (SARS-CoV-2) causing COVID-19 disease
spreads rapidly in the world. Rapid and early detection of SARS-CoV-2 facilitates early …
spreads rapidly in the world. Rapid and early detection of SARS-CoV-2 facilitates early …
[HTML][HTML] How to measure and evaluate binding affinities
I Jarmoskaite, I AlSadhan, PP Vaidyanathan… - Elife, 2020 - elifesciences.org
Quantitative measurements of biomolecule associations are central to biological
understanding and are needed to build and test predictive and mechanistic models. Given …
understanding and are needed to build and test predictive and mechanistic models. Given …
[HTML][HTML] Mechanistic Insights into the cis-and trans-Acting DNase Activities of Cas12a
Summary CRISPR-Cas12a (Cpf1) is an RNA-guided DNA-cutting nuclease that has been
repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target …
repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target …
[HTML][HTML] Mapping the genetic landscape of DNA double-strand break repair
Cells repair DNA double-strand breaks (DSBs) through a complex set of pathways critical for
maintaining genomic integrity. To systematically map these pathways, we developed a high …
maintaining genomic integrity. To systematically map these pathways, we developed a high …
TnpB structure reveals minimal functional core of Cas12 nuclease family
G Sasnauskas, G Tamulaitiene, G Druteika, A Carabias… - Nature, 2023 - nature.com
The widespread TnpB proteins of IS200/IS605 transposon family have recently emerged as
the smallest RNA-guided nucleases capable of targeted genome editing in eukaryotic cells …
the smallest RNA-guided nucleases capable of targeted genome editing in eukaryotic cells …
[HTML][HTML] CRISPR-Cas tools and their application in genetic engineering of human stem cells and organoids
CRISPR-Cas technology has revolutionized biological research and holds great therapeutic
potential. Here, we review CRISPR-Cas systems and their latest developments with an …
potential. Here, we review CRISPR-Cas systems and their latest developments with an …
[HTML][HTML] All-in-one dual CRISPR-Cas12a (AIOD-CRISPR) assay: a case for rapid, ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 and HIV virus
A recent outbreak of novel coronavirus (SARS-CoV-2), the causative agent of COVID-19,
has spread rapidly all over the world. Human immunodeficiency virus (HIV) is another …
has spread rapidly all over the world. Human immunodeficiency virus (HIV) is another …
Enzyme kinetics and detector sensitivity determine limits of detection of amplification-free CRISPR-Cas12 and CRISPR-Cas13 diagnostics
DA Huyke, A Ramachandran, VI Bashkirov… - Analytical …, 2022 - ACS Publications
Interest in CRISPR-Cas12 and CRISPR-Cas13 detection continues to increase as these
detection schemes enable the specific recognition of nucleic acids. The fundamental …
detection schemes enable the specific recognition of nucleic acids. The fundamental …