Publisher Summary This chapter discusses the structure and properties of the cell surface
coat. Cell surface coats have been found on all living cells that have been examined with …

Verapamil, an agent known rapidly to block calcium uptake into islets of Langerhans, has
been used to study the roles of intra-and extracellular calcium in the two phases of glucose …

The regulation of intracellular calcium is certainly one of the fields of biology most intensely
and continuously investigated during the last 20 years. A firm conclusion that emerges from …

Density gradient centrifugation techniques, using iso-osmotic colloidal silica suspensions
(Percoll), were developed for the isolation of insulin secretory granules from a transplantable …

Publisher Summary This chapter discusses the methods of measuring intracellular calcium.
The techniques described offer a wide range of approaches for assaying intracellular Ca 2+ …

The distribution of calcium and magnesium has been studied in the acinar cells of the
pancreas of the guinea pig. Most of the magnesium was found to be associated with the …

Fixation of rat parotid with an unbuffered osmium tetroxide solution containing nearly
saturated potassium (pyro) antimonate resulted in abundant deposition of cation-antimonate …

1. Fluxes of 45Ca2+ were studied in pancreatic islets from non‐inbred ob/ob‐mice. Because
La3+ blocked the transmembrane fluxes of 45Ca2+ in islet cells, incubations aimed at …

Giant axons from the squid, Loligo pealei, were fixed in glutaraldehyde and postfixed in
osmium tetroxide. Calcium chloride (5 mM/liter) was added to all aqueous solutions used for …

1. The release of 45calcium from prelabelled pancreatic islets is rapidly and almost totally
inhibited by lanthanum. 2. Glucose provokes an intitial fall followed by a secondary rise in …