A comparative analysis of metabolism and viability in porcine oocytes during in vitro maturation

RL Krisher, AM Brad, JR Herrick, ML Sparman… - Animal reproduction …, 2007 - Elsevier
The importance of oocyte quality cannot be overstated, because it impacts all subsequent
events during development of the embryo, the fetus and even the resulting offspring. Oocyte …

The current status of oocyte recovery, in vitro embryo production, and embryo transfer in domestic animals, with an emphasis on the bovine

JF Hasler - Journal of Animal Science, 1998 - academic.oup.com
An intense interest exists internationally in investigating in vitro procedures for the
maturation, fertilization, and culture of immature oocytes from domestic mammalian species …

Developmental competence of prepubertal and adult goat oocytes cultured in semi-defined media following laparoscopic recovery

J Koeman, CL Keefer, H Baldassarre, BR Downey - Theriogenology, 2003 - Elsevier
With an increased interest in transgenic animal production, the caprine species offers many
advantages, and the prepubertal goat is a potential source of large numbers of oocytes for in …

Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

E Rodríguez-González, M López-Bejar… - Reproduction …, 2003 - rnd.edpsciences.org
The aim of this study was to assess the effect of oocyte selection using the brilliant cresyl
blue (BCB) test plus the addition of cysteamine to the in vitro maturation (IVM) medium to …

Effect of culture media on embryo development from prepubertal goat IVM-IVF oocytes

D Izquierdo, P Villamediana, MT Paramio - Theriogenology, 1999 - Elsevier
Experiments were carried out to develop and improve in vitro culture systems for IVM-IVF
prepubertal goat oocytes. Cumulus oocyte complexes (COC) were obtained by slicing …

Effect of semen preparation on IVF of prepubertal goat oocytes

MJ Palomo, D Izquierdo, T Mogas, MT Paramio - Theriogenology, 1999 - Elsevier
The aim of these experiments was to study the effects of different methods of washing and
selection of spermatozoa on the IVF of IVM oocytes from prepubertal goats. Fresh ejaculates …

Transgenic goats produced by DNA pronuclear microinjection of in vitro derived zygotes

B Wang, H Baldassarre, T Tao… - Molecular …, 2002 - Wiley Online Library
This study was undertaken to investigate various factors affecting the outcomes of in vitro
fertilization (IVF) of oocytes retrieved by laparoscopic ovum pick‐up (LOPU) technique from …

Effect of macromolecule supplementation during in vitro maturation of goat oocytes on developmental potential

JR Herrick, E Behboodi, E Memili… - Molecular …, 2004 - Wiley Online Library
In vitro maturation (IVM) of goat oocytes with serum‐supplemented media results in oocytes
with reduced developmental potential. The objective of this study was to develop a defined …

Supplementation with cysteamine during maturation and embryo culture on embryo development of prepubertal goat oocytes selected by the brilliant cresyl blue test

A Urdaneta, AR Jiménez-Macedo, D Izquierdo… - Zygote, 2003 - cambridge.org
Our previous studies have shown that the addition of 100 μM cysteamine to the in vitro
maturation (IVM) medium increased the embryo development of prepubertal goat oocytes …

Caprine blastocyst development after in vitro fertilization with spermatozoa frozen in different extenders

L Keskintepe, AA Simplicio, BG Brackett - Theriogenology, 1998 - Elsevier
The feasibility of using frozen-thawed semen in caprine IVF outside the breeding season
was investigated. Electroejaculated spermatozoa from a Nubian buck were washed twice …