The development of new ion-activation/dissociation methods continues to be one of the most
active areas of mass spectrometry owing to the broad applications of tandem mass …

Ammonium acetate (NH4Ac) is a widely used solvent additive in native electrospray
ionization (ESI) mass spectrometry. NH4Ac can undergo proton transfer to form ammonia …

Native mass spectrometry (MS) allows the interrogation of structural aspects of
macromolecules in the gas phase, under the premise of having initially maintained their …

Ion mobility mass spectrometry (IM-MS) allows separation of native protein ions into
“conformational families”. Increasing the IM resolving power should allow finer structural …

Experiments and molecular dynamics (MD) simulations in the literature indicate that
gaseous proteins generated by electrospray ionization (ESI) can retain native-like structures …

Proteins can adopt different conformational states that are important for their biological
function and, in some cases, can be responsible for their dysfunction. The essential roles …

The mechanisms whereby protein ions are released into the gas phase from charged
droplets during electrospray ionization (ESI) continue to be controversial. Several pathways …

The ion evaporation model (IEM) and the charged residue model (CRM) represent
cornerstones of any discussion related to the mechanism of electrospray ionization (ESI) …

Characterizing structures of protein complexes and their disease-related aberrations is
essential to understanding molecular mechanisms of many biological processes …

The isolation of biomolecules in a high vacuum enables experiments on fragile species in
the absence of a perturbing environment. Since many molecular properties are influenced …