CRISPR/Cas genome editing and precision plant breeding in agriculture

K Chen, Y Wang, R Zhang, H Zhang… - Annual review of plant …, 2019 - annualreviews.org
Enhanced agricultural production through innovative breeding technology is urgently
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …

Novel CRISPR–Cas systems: an updated review of the current achievements, applications, and future research perspectives

S Nidhi, U Anand, P Oleksak, P Tripathi, JA Lal… - International journal of …, 2021 - mdpi.com
According to Darwin's theory, endless evolution leads to a revolution. One such example is
the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)–Cas system, an …

Plant gene editing through de novo induction of meristems

MF Maher, RA Nasti, M Vollbrecht, CG Starker… - Nature …, 2020 - nature.com
Plant gene editing is typically performed by delivering reagents such as Cas9 and single
guide RNAs to explants in culture. Edited cells are then induced to differentiate into whole …

Engineering crops of the future: CRISPR approaches to develop climate-resilient and disease-resistant plants

SSA Zaidi, A Mahas, H Vanderschuren, MM Mahfouz - Genome biology, 2020 - Springer
To meet increasing global food demand, breeders and scientists aim to improve the yield
and quality of major food crops. Plant diseases threaten food security and are expected to …

CRISPR for crop improvement: an update review

D Jaganathan, K Ramasamy, G Sellamuthu… - Frontiers in plant …, 2018 - frontiersin.org
The availability of genome sequences for several crops and advances in genome editing
approaches has opened up possibilities to breed for almost any given desirable trait …

Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs

EE Ellison, U Nagalakshmi, ME Gamo, P Huang… - Nature plants, 2020 - nature.com
An in planta gene editing approach was developed wherein Cas9 transgenic plants are
infected with an RNA virus that expresses single guide RNAs (sgRNAs). The sgRNAs are …

Navigating complexity to breed disease-resistant crops

R Nelson, T Wiesner-Hanks, R Wisser… - Nature Reviews …, 2018 - nature.com
Plant diseases are responsible for substantial crop losses each year and pose a threat to
global food security and agricultural sustainability. Improving crop resistance to pathogens …

Development of broad virus resistance in non‐transgenic cucumber using CRISPR/Cas9 technology

J Chandrasekaran, M Brumin, D Wolf… - Molecular plant …, 2016 - Wiley Online Library
Genome editing in plants has been boosted tremendously by the development of
CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) technology …

CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding

JN Tripathi, VO Ntui, M Ron, SK Muiruri, A Britt… - Communications …, 2019 - nature.com
Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of
plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV …

Progress and prospects in plant genome editing

K Yin, C Gao, JL Qiu - Nature plants, 2017 - nature.com
The emergence of sequence-specific nucleases that enable genome editing is
revolutionizing basic and applied biology. Since the introduction of CRISPR–Cas9, genome …