[HTML][HTML] Emergent CRISPR–Cas-based technologies for engineering non-model bacteria
Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …
[HTML][HTML] Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy
M Chehelgerdi, M Chehelgerdi… - Molecular cancer, 2024 - Springer
The CRISPR system is a revolutionary genome editing tool that has the potential to
revolutionize the field of cancer research and therapy. The ability to precisely target and edit …
revolutionize the field of cancer research and therapy. The ability to precisely target and edit …
Engineering plants using diverse CRISPR-associated proteins and deregulation of genome-edited crops
The CRISPR/Cas system comprises RNA-guided nucleases, the target specificity of which is
directed by Watson–Crick base pairing of target loci with single guide (sg) RNA to induce the …
directed by Watson–Crick base pairing of target loci with single guide (sg) RNA to induce the …
New possibilities for trait improvement via mobile CRISPR-RNA
CRISPR/Cas9 gene technology is transported as RNA from transgenic roots to distal parts of
unmodified grafted scion, where it is translated into proteins to induce heritable mutagenesis …
unmodified grafted scion, where it is translated into proteins to induce heritable mutagenesis …
Tracking-seq reveals the heterogeneity of off-target effects in CRISPR–Cas9-mediated genome editing
M Zhu, R Xu, J Yuan, J Wang, X Ren, T Cong… - Nature …, 2024 - nature.com
The continued development of novel genome editors calls for a universal method to analyze
their off-target effects. Here we describe a versatile method, called Tracking-seq, for in situ …
their off-target effects. Here we describe a versatile method, called Tracking-seq, for in situ …
[HTML][HTML] High-resolution genome-wide mapping of chromosome-arm-scale truncations induced by CRISPR–Cas9 editing
Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated
protein 9 (Cas9) is a powerful tool for introducing targeted mutations in DNA, but recent …
protein 9 (Cas9) is a powerful tool for introducing targeted mutations in DNA, but recent …
[HTML][HTML] CRISPR/Cas9-mediated gene therapy for glioblastoma: a scoping review
This scoping review examines the use of CRISPR/Cas9 gene editing in glioblastoma (GBM),
a predominant and aggressive brain tumor. Categorizing gene targets into distinct groups …
a predominant and aggressive brain tumor. Categorizing gene targets into distinct groups …
Prospects and challenges of CRISPR/Cas9 gene‐editing technology in cancer research
L Ning, J Xi, Y Zi, M Chen, Q Zou, X Zhou… - Clinical …, 2023 - Wiley Online Library
Cancer, one of the leading causes of death, usually commences and progresses as a result
of a series of gene mutations and dysregulation of expression. With the development of …
of a series of gene mutations and dysregulation of expression. With the development of …
CRISPR/Cas9-mediated base editors and their prospects for mitochondrial genome engineering
S Eghbalsaied, C Lawler, B Petersen, RA Hajiyev… - Gene Therapy, 2024 - nature.com
Base editors are a type of double-stranded break (DSB)-free gene editing technology that
has opened up new possibilities for precise manipulation of mitochondrial DNA (mtDNA) …
has opened up new possibilities for precise manipulation of mitochondrial DNA (mtDNA) …
[HTML][HTML] Tumor biomarkers for diagnosis, prognosis and targeted therapy
Y Zhou, L Tao, J Qiu, J Xu, X Yang, Y Zhang… - … and Targeted Therapy, 2024 - nature.com
Tumor biomarkers, the substances which are produced by tumors or the body's responses to
tumors during tumorigenesis and progression, have been demonstrated to possess critical …
tumors during tumorigenesis and progression, have been demonstrated to possess critical …