Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

The CRISPR system is a revolutionary genome editing tool that has the potential to
revolutionize the field of cancer research and therapy. The ability to precisely target and edit …

The CRISPR/Cas system comprises RNA-guided nucleases, the target specificity of which is
directed by Watson–Crick base pairing of target loci with single guide (sg) RNA to induce the …

CRISPR/Cas9 gene technology is transported as RNA from transgenic roots to distal parts of
unmodified grafted scion, where it is translated into proteins to induce heritable mutagenesis …

The continued development of novel genome editors calls for a universal method to analyze
their off-target effects. Here we describe a versatile method, called Tracking-seq, for in situ …

Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated
protein 9 (Cas9) is a powerful tool for introducing targeted mutations in DNA, but recent …

This scoping review examines the use of CRISPR/Cas9 gene editing in glioblastoma (GBM),
a predominant and aggressive brain tumor. Categorizing gene targets into distinct groups …

Cancer, one of the leading causes of death, usually commences and progresses as a result
of a series of gene mutations and dysregulation of expression. With the development of …

Base editors are a type of double-stranded break (DSB)-free gene editing technology that
has opened up new possibilities for precise manipulation of mitochondrial DNA (mtDNA) …

Tumor biomarkers, the substances which are produced by tumors or the body's responses to
tumors during tumorigenesis and progression, have been demonstrated to possess critical …