The CRISPR/Cas system comprises RNA-guided nucleases, the target specificity of which is
directed by Watson–Crick base pairing of target loci with single guide (sg) RNA to induce the …

Food security and crop production are challenged worldwide due to overpopulation,
changing environmental conditions, crop establishment failure, and various kinds of post …

Sweet orange (Citrus sinensis) is the most economically important species for the citrus
industry. However, it is susceptible to many diseases including citrus bacterial canker …

Gene editing using clustered regularly interspaced short palindromic repeat/CRISPR-
associated proteins (CRISPR/Cas) has revolutionized biotechnology and provides genetic …

The major challenges that agriculture is facing in the twenty-first century are increasing
droughts, water scarcity, flooding, poorer soils, and extreme temperatures due to climate …

Prime editing (PE) is a precise genome manipulation technology based on the “search and
replace” approach of the CRISPR-Cas9 system, while it does not require the exogenous …

CRISPR/Cas-based genome editing technologies, which allow the precise manipulation of
plant genomes, have revolutionized plant science and enabled the creation of germplasms …

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated
protein (Cas)-mediated gene disruption has revolutionized biomedical research as well as …

PAM‐relaxed Cas9 nucleases, cytosine base editors and adenine base editors are
promising tools for precise genome editing in plants. However, their genome‐wide off‐target …

CRISPR/Cas-mediated genome editing is a powerful tool of plant functional genomics. Hairy
root transformation is a rapid and convenient approach for obtaining transgenic roots. When …