[HTML][HTML] Molecular characterization and protective efficacy of vacuolar protein sorting 29 from Eimeria tenella

T Shi, T Li, Y Hao, H Sun, Y Fu, W Yan… - Frontiers in Cellular and …, 2023 - ncbi.nlm.nih.gov
Methods Here, we cloned and prokaryotically expressed a partial sequence of Eimeria
tenella VPS29 (EtVPS29) with RT-PCR and engineered strain of Escherichia coli …

[引用][C] In silico analysis of different signal peptides to discover a panel of appropriate signal peptides for secretory production of Interferon-beta 1b in Escherichia coli

S Ghovvati, Z Pezeshkian… - Acta Biochimica …, 2018 - ojs.ptbioch.edu.pl
Signal peptides (SPs) are one of the most important factors for suitable secretion of the
recombinant heterologous proteins in Escherichia coli (E. coli). The objective of this study …

[HTML][HTML] The goat β‑casein/CMV chimeric promoter drives the expression of hLF in transgenic goats produced by cell transgene microinjection

T Zhang, Y Yuan, R Lu, S Xu… - International …, 2019 - spandidos-publications.com
There is growing interest in the application of lactoferrin (LF) as a drug or food additive for
animals and humans. The objective of this study was to produce transgenic cloned goats …

Ethanol as additive enhances expression of Ranibizumab in Escherichia coli: Impact on cellular physiology and transcriptome

P Priyanka, RS Patil, P Meshram, JA Gupta… - Process …, 2022 - Elsevier
Ranibizumab is an antibody fragment used for treatment of blurred vision due to age-related
macular degeneration. Most manufacturers currently express it in Escherichia coli BL21 …

Recombinant active peptides and their therapeutic functions

YS Ajingi, N Rukying, A Aroonsri… - Current Pharmaceutical …, 2022 - ingentaconnect.com
Recombinant active peptides are utilized as diagnostic and biotherapeutics in various
maladies and as bacterial growth inhibitors in the food industry. This consequently …

Identification of critical chemical modifications by size exclusion chromatography of stressed antibody-target complexes with competitive binding

RL Shi, G Xiao, TM Dillon, A McAuley, MS Ricci… - MAbs, 2021 - Taylor & Francis
Chemical modifications (attributes) in the binding regions of stressed therapeutic proteins
may affect binding to target and efficacy of therapeutic proteins. The method presented here …

Holin‐assisted bacterial recombinant protein export

T Guo, Y Cui, L Zhang, X Xu, Z Xu… - Biotechnology and …, 2022 - Wiley Online Library
A simple generic method for enhancing extracellular protein yields in engineered bacteria is
still lacking. Here, we demonstrated that phage‐encoded holin can be used to export …

Mammalian cell lines used in bioprocessing

N ERGEN, H TÜFEKÇİ - Journal of Experimental and Clinical …, 2022 - dergipark.org.tr
Various number of expression and host systems are used in biologics manufacturing. The
most commonly preferred systems are based on bacteria, yeast, mammalian cells, insect …

Enzymatic technologies as green and sustainable techniques for remediation of oil-contaminated environment: state of the art

JO Osuoha, EO Nwaichi - International Journal of Environmental Science …, 2021 - Springer
The consistent discharge of xenobiotic compounds including hydrocarbon-containing
substances, pesticides, plastics, fertilizers, etc., in the surrounding is the main source of …

Recent developments in systems biology and genetic engineering toward design of vaccines for TB

Sunita, Y Singh, G Beamer, X Sun… - Critical Reviews in …, 2022 - Taylor & Francis
Tuberculosis (TB) is one of the most prevalent diseases worldwide. The currently available
Bacillus Calmette-Guérin vaccine is not sufficient in protecting against pulmonary TB …