Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …

CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

Growing environmental concern sparks renewed interest in the sustainable production of
(bio) materials that can replace oil‐derived goods. Polyhydroxyalkanoates (PHAs) are …

Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

The cryptic secondary metabolite biosynthetic gene clusters (BGCs) far outnumber currently
known secondary metabolites. Heterologous production of secondary metabolite BGCs in …

Fluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture
and materials. Addition of fluorine to organic structures represents a unique strategy for …

Pseudomonas putida is a microbial chassis of huge potential for industrial and
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …

Major advances toward a bio-based industry enabled cost-efficient bioproduction of multiple
chemicals, yet successful industrial processes are relatively scarce and limited to the use of …