The human history has witnessed the rapid development of technologies such as high-
throughput sequencing and mass spectrometry that led to the concept of 'omics' and …

Adult stem cells are important for mammalian tissues, where they act as a cell reserve that
supports normal tissue turnover and can mount a regenerative response following acute …

Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for
transcriptome-wide analysis of differential gene expression and differential splicing of …

Most human genes have multiple sites at which RNA 3ʹ end cleavage and polyadenylation
can occur, enabling the expression of distinct transcript isoforms under different conditions …

Alternative polyadenylation (APA) is an RNA-processing mechanism that generates distinct
3′ termini on mRNAs and other RNA polymerase II transcripts. It is widespread across all …

3′-untranslated regions (3′-UTRs) are the noncoding parts of mRNAs. Compared to
yeast, in humans, median 3′-UTR length has expanded approximately tenfold alongside …

Deep sequencing has been revolutionizing biology and medicine in recent years, providing
single base‐level precision for our understanding of nucleic acid sequences in high …

We adapted UV CLIP (cross-linking immunoprecipitation) to accurately locate tens of
thousands of m6A residues in mammalian mRNA with single-nucleotide resolution. More …

Alternative polyadenylation (APA) is a pervasive mechanism in the regulation of most
human genes, and its implication in diseases including cancer is only beginning to be …

We evaluated 25 protocol variants of 14 independent computational methods for exon
identification, transcript reconstruction and expression-level quantification from RNA-seq …