The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …

The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …

The spontaneous deamination of cytosine is a major source of transitions from C• G to T• A
base pairs, which account for half of known pathogenic point mutations in humans. The …

The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

We recently developed base editing, the programmable conversion of target C: G base pairs
to T: A without inducing double-stranded DNA breaks (DSBs) or requiring homology …

Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new
CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or …

Genome editing has transformed the life sciences and has exciting prospects for use in
treating genetic diseases. Our laboratory developed base editing to enable precise and …

The emergence of sequence-specific nucleases that enable genome editing is
revolutionizing basic and applied biology. Since the introduction of CRISPR–Cas9, genome …

CRISPR–Cas9 methods have been applied to generate random insertions and deletions,
large deletions, targeted insertions or replacements of short sequences, and precise base …

Abiotic stresses, including drought, salinity, temperature, and heavy metals, pose a major
challenge for crop production and cause substantial yield reduction worldwide. Breeding …