Poly (A) tails have long been known as stable 3′ modifications of eukaryotic mRNAs,
added during nuclear pre‐mRNA processing. It is now appreciated that this modification is …

Gene expression universally relies on protein synthesis, where ribosomes recognize and
decode the messenger RNA template by cycling through translation initiation, elongation …

Single-cell RNA sequencing (scRNA-seq) has become an established and powerful method
to investigate transcriptomic cell-to-cell variation, thereby revealing new cell types and …

The polyadenosine tail (poly [A]-tail) is a universal modification of eukaryotic messenger
RNAs (mRNAs) and non-coding RNAs (ncRNAs). In budding yeast, Pap1-synthesized …

Message RNA poly (A) tails are vital for their function and regulation. However, the full-
length sequence of mRNA isoforms with their poly (A) tails remains undetermined. Here, we …

Eukaryotic genomes generate a heterogeneous ensemble of mRNAs and long noncoding
RNAs (lncRNAs). LncRNAs and mRNAs are both transcribed by Pol II and acquire 5′ caps …

Highlights•Integrated structural biology approaches have provided new insights into the
mechanism of eukaryotic mRNA 3′-end processing.•The polymerase modules of yeast and …

Termination of transcription by RNA polymerase II requires two distinct processes: The
formation of a defined 3′ end of the transcribed RNA, as well as the disengagement of RNA …

The nuclear export of mRNA, in which Mex67–Mtr2 mediates movement of mature
transcripts through nuclear pores, represents the culmination of the nuclear portion of the …

During their synthesis in the cell nucleus, most eukaryotic mRNAs undergo a two‐step 3′‐
end processing reaction in which the pre‐mRNA is cleaved and released from the …