BACKGROUND Although laboratory procedures, along with culture media formulations,
have improved over the past two decades, the issue remains that human IVF is performed in …

In human in-vitro fertilization (IVF) embryos are routinely transferred to the uterus on day 2 or
day 3 of development. Resultant implantation and pregnancy rates are disappointingly low …

The aim of this study was to develop a serum-free culture system that could support high
levels of cleavage and blastocyst formation from sheep zygotes developed in vitro. To this …

The first edition of the Textbook of Assisted Reproductive Techniques was published in
2001. As the textbook now enters its sixth edition, some 45 years since the birth of Louise …

Culture of mammalian zygotes individually and in small groups results in lower
developmental rates than culture of large groups. Zona‐free zygotes also have impaired …

We have recently reported the use of sequential simplex optimization methods to design a
medium (SOM) that overcomes the block to development beyond two cells which occurs in …

Cell death is a widespread feature in the blastocysts of many mammals. Isolated cells in
both the inner cell mass and the trophectoderm undergo cell death. These dying cells …

The success rate of human in-vitro fertilization (IVF) remains low, with only-10% of embryos
transferred resulting in a term pregnancy. A major contributor to this embryonic loss Is poor …

In the presence of cycloheximide (CHX) to inhibit protein synthesis, a high concentration of
staurosporine (STS) induces almost all cells in explant cultures of 8/8 types of newborn …

The presence of ammonium in the culture medium has significant detrimental effects on the
regulation of embryo physiology and genetics. Ammonium levels build up linearly over time …