Abstract Polyadenylation [poly (A)] signals (PAS) are a defining feature of eukaryotic protein-
coding genes. The central sequence motif AAUAAA was identified in the mid-1970s and …

Formation of mRNA 3′ ends in eukaryotes requires the interaction of transacting factors
with cis-acting signal elements on the RNA precursor by two distinct mechanisms, one for …

Transgenesis is an important tool for assessing gene function. In zebrafish, transgenesis has
suffered from three problems: the labor of building complex expression constructs using …

The expression of genes delivered by retroviral vectors is often inefficient, a potential
obstacle for their widespread use in human gene therapy. Here, we explored the possibility …

The ability to specify the expression levels of exogenous genes inserted in the genomes of
transgenic animals is critical for the success of a wide variety of experimental manipulations …

Many genes have been described and characterized which result in alternative
polyadenylation site use at the 3′-end of their mRNAs based on the cellular environment …

To test whether the hypothesis that the Alzheimer's Aβ peptide is neurotoxic, we introduced
a transgene into mice to direct expression of this peptide to neurons. We show that the …

Association of alleles at the Taq l A, Taq l B, intron 6, Taq l D, exon 7, exon 8, and promoter-
141C sites of the D2 dopamine receptor gene with D2 dopamine receptor binding …

Monoclonal antibodies (mAbs) are currently used for many diagnostic and therapeutic
applications. The high demand for these biopharmaceuticals has led to the development of …

Active LINE-1 (L1) elements possess the ability to transduce non-L1 DNA flanking their 3′
ends to new genomic locations. Occasionally, the 3′ end processing machinery may …