[HTML][HTML] Ending the message: poly (A) signals then and now
NJ Proudfoot - Genes & development, 2011 - genesdev.cshlp.org
Abstract Polyadenylation [poly (A)] signals (PAS) are a defining feature of eukaryotic protein-
coding genes. The central sequence motif AAUAAA was identified in the mid-1970s and …
coding genes. The central sequence motif AAUAAA was identified in the mid-1970s and …
Formation of mRNA 3′ ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis
J Zhao, L Hyman, C Moore - Microbiology and molecular biology …, 1999 - Am Soc Microbiol
Formation of mRNA 3′ ends in eukaryotes requires the interaction of transacting factors
with cis-acting signal elements on the RNA precursor by two distinct mechanisms, one for …
with cis-acting signal elements on the RNA precursor by two distinct mechanisms, one for …
The Tol2kit: A multisite gateway‐based construction kit for Tol2 transposon transgenesis constructs
KM Kwan, E Fujimoto, C Grabher… - … dynamics: an official …, 2007 - Wiley Online Library
Transgenesis is an important tool for assessing gene function. In zebrafish, transgenesis has
suffered from three problems: the labor of building complex expression constructs using …
suffered from three problems: the labor of building complex expression constructs using …
Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors
The expression of genes delivered by retroviral vectors is often inefficient, a potential
obstacle for their widespread use in human gene therapy. Here, we explored the possibility …
obstacle for their widespread use in human gene therapy. Here, we explored the possibility …
Using translational enhancers to increase transgene expression in Drosophila
BD Pfeiffer, JW Truman… - Proceedings of the …, 2012 - National Acad Sciences
The ability to specify the expression levels of exogenous genes inserted in the genomes of
transgenic animals is critical for the success of a wide variety of experimental manipulations …
transgenic animals is critical for the success of a wide variety of experimental manipulations …
Alternative poly (A) site selection in complex transcription units: means to an end?
G Edwalds-Gilbert, KL Veraldi… - Nucleic acids …, 1997 - academic.oup.com
Many genes have been described and characterized which result in alternative
polyadenylation site use at the 3′-end of their mRNAs based on the cellular environment …
polyadenylation site use at the 3′-end of their mRNAs based on the cellular environment …
The Alzheimer's Aβ peptide induces neurodegeneration and apoptotic cell death in transgenic mice
FM LaFerla, BT Tinkle, CJ Bieberich… - Nature …, 1995 - nature.com
To test whether the hypothesis that the Alzheimer's Aβ peptide is neurotoxic, we introduced
a transgene into mice to direct expression of this peptide to neurons. We show that the …
a transgene into mice to direct expression of this peptide to neurons. We show that the …
Association of seven polymorphisms of the D2 dopamine receptor gene with brain receptor-binding characteristics
T Ritchie, EP Noble - Neurochemical research, 2003 - Springer
Association of alleles at the Taq l A, Taq l B, intron 6, Taq l D, exon 7, exon 8, and promoter-
141C sites of the D2 dopamine receptor gene with D2 dopamine receptor binding …
141C sites of the D2 dopamine receptor gene with D2 dopamine receptor binding …
Guidelines to cell engineering for monoclonal antibody production
AR Costa, ME Rodrigues, M Henriques… - European Journal of …, 2010 - Elsevier
Monoclonal antibodies (mAbs) are currently used for many diagnostic and therapeutic
applications. The high demand for these biopharmaceuticals has led to the development of …
applications. The high demand for these biopharmaceuticals has led to the development of …
Transduction of 3′-flanking sequences is common in L1 retrotransposition
JL Goodier, EM Ostertag… - Human molecular …, 2000 - academic.oup.com
Active LINE-1 (L1) elements possess the ability to transduce non-L1 DNA flanking their 3′
ends to new genomic locations. Occasionally, the 3′ end processing machinery may …
ends to new genomic locations. Occasionally, the 3′ end processing machinery may …