[HTML][HTML] Sars-Cov-2 detection methods

A Lino, MA Cardoso, HMR Gonçalves, P Martins-Lopes - Chemosensors, 2022 - mdpi.com
A fast and highly specific detection of COVID-19 infections is essential in managing the virus
dissemination networks. The most relevant technologies developed for SARS-CoV-2 …

[PDF][PDF] Guidance for surveillance of SARS-CoV-2 variants: interim guidance, 9 August 2021

World Health Organization - 2021 - apps.who.int
Background SARS-CoV-2 is an enveloped, positive-sense single-stranded RNA virus with a
30-kilobase genome, which, like all viruses, accumulates nucleotide mutations over time …

RT-qPCR assays for SARS-CoV-2 variants of concern in wastewater reveals compromised vaccination-induced immunity

K Yaniv, E Ozer, Y Lewis, A Kushmaro - Water research, 2021 - Elsevier
SARS-CoV-2 variants of concern, demonstrating higher infection rate and lower vaccine
effectiveness as compared with the original virus, are important factors propelling the …

[HTML][HTML] Nationwide harmonization effort for semi-quantitative reporting of SARS-CoV-2 PCR test results in Belgium

L Cuypers, J Bode, K Beuselinck, L Laenen… - Viruses, 2022 - mdpi.com
From early 2020, a high demand for SARS-CoV-2 tests was driven by several testing
indications, including asymptomatic cases, resulting in the massive roll-out of PCR assays to …

[HTML][HTML] Rapid evolution of SARS-CoV-2 challenges human defenses

CM Duarte, DI Ketcheson, VM Eguíluz, S Agustí… - Scientific Reports, 2022 - nature.com
The race between pathogens and their hosts is a major evolutionary driver, where both
reshuffle their genomes to overcome and reorganize the defenses for infection, respectively …

Impact of SARS-CoV-2 variants on the analytical sensitivity of rRT-PCR assays

Y Chen, Y Han, J Yang, Y Ma, J Li… - Journal of clinical …, 2022 - Am Soc Microbiol
Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with
enhanced transmissibility, pathogenicity, and immune escape ability have ravaged many …

[HTML][HTML] SARS-CoV2 RT-PCR assays: In vitro comparison of 4 WHO approved protocols on clinical specimens and its implications for real laboratory practice through …

M Gdoura, I Abouda, M Mrad, I Ben Dhifallah, Z Belaiba… - Virology Journal, 2022 - Springer
Introduction RT-PCR testing on nasopharyngeal swabs is a key component in the COVID-19
fighting, provided to use sensitive and specific SARS-CoV2 genome targets. In this study, we …

A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2

H Wang, S Jean, SA Wilson, JM Lucyshyn… - … and Infectious Disease, 2022 - Elsevier
Abstract One SARS-CoV-2-positive sample demonstrated impaired detection of the N1
target by RT-PCR using US CDC primer/probe sets. A 3 nucleotide deletion was discovered …

Robust clinical detection of SARS‐CoV‐2 variants by RT‐PCR/MALDI‐TOF multitarget approach

MM Hernandez, R Banu… - Journal of medical …, 2022 - Wiley Online Library
Abstract The coronavirus disease 2019 (COVID‐19) pandemic has sparked the rapid
development of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) …

[HTML][HTML] Rapid detection of the widely circulating B. 1.617. 2 (Delta) SARS-CoV-2 variant

AE Rosato, E Msiha, B Weng, M Mesisca, R Gnass… - Pathology, 2022 - Elsevier
The emergence of the B. 1.617. 2 (Delta) variant of the severe acute syndrome coronavirus
(SARS-CoV-2) that emerged in 2019 (COVID-19), resulted in a surge of cases in India and …