The production and characterization of an active recombinant N-acetylgalactosamine-6-
sulfate sulfatase (GALNS) in Escherichia coli BL21 (DE3) has been previously reported. In …

Abstract Pectate lyase (EC 4.2. 2.2) gene from Bacillus subtilis RCK was cloned and
expressed in Escherichia coli to maximize its production. In addition to soluble fraction …

Escherichia coli is one of the most commonly used organisms for producing recombinant
protein. The recognized cell genome and well-known cell factory of the bacteria make it an …

Abstract Background Inclusion bodies (IBs) were generally considered to be inactive protein
deposits and did not hold any attractive values in biotechnological applications. Recently …

Background Production of recombinant proteins in bacterial hosts often produces insoluble
intracellular particles called inclusion bodies. Recovery of active protein from inclusion …

Aggregation of proteins is at the nexus of molecular processes crucial to aging, disease, and
employing proteins for biotechnology and medical applications. There has been much …

Neuraminidase (NA) is one of the targets for the development of new antivirals against the
influenza virus. The recombinant Escherichia coli cells, namely the strains BL21 (DE3) …

Conventional refolding methods are associated with low yields due to misfolding and high
aggregation rates or very dilute proteins. In this study, we describe the optimization of the …

Bacteria became the primary workhorses of the biotechnology industry due to fast growth on
inexpensive media, well understood genetics, and an advanced genetic engineering toolset …

[eng] In 2015 our research group described, in P. aeruginosa, the oleate-diol synthase
system which consists of two sequentially and independently enzymatic reactions: 10S …