Climate change imposes a severe threat to agricultural systems, food security, and human
nutrition. Meanwhile, efforts in crop and livestock gene editing have been undertaken to …

The prospect of genome editing offers a number of promising opportunities for livestock
breeders. Firstly, these tools can be used in functional genomics to elucidate gene function …

CRISPR/Cas9 technology has transformed mouse genome editing with unprecedented
precision, efficiency, and ease; however, the current practice of microinjecting CRISPR …

Genetically engineered mouse models harboring large sequence insertions or modifications
are critical for a wide range of applications including endogenous gene tagging, conditional …

In this report, we present an improved protocol for CRISPR/Cas9 genome editing in mice.
The procedure consists in the electroporation of intact mouse zygotes with ribonucleoprotein …

Background Xenoantigens are a major source of concern with regard to the success of
interspecific xenografts. GGTA1 encodes α1, 3-galactosyltransferase, which is essential for …

Beef and mutton production has been aided by breeding to integrate allelic diversity for
myostatin (MSTN), but a lack of diversity in the MSTN germplasm has limited similar …

Conditional knockout using Cre/lox is essential for functional analysis of genes.
CRISPR/Cas in combination with two sets of guide RNAs and a single-stranded …

Genetically modified animals, especially rodents, are widely used in biomedical research.
However, non-rodent models are required for efficient translational medicine and preclinical …

Germline editing, the process by which the genome of an individual is edited in such a way
that the change is heritable, has been applied to a wide variety of animals [DA Sorrell, AF …