Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Cells from all three domains of life, Archaea, Bacteria and Eukarya, produce extracellular
vesicles (EVs) which are sometimes associated with filamentous structures known as …

Advances in imaging technologies have revealed that many bacteria possess organelles
with a proteomically defined lumen and a macromolecular boundary. Some are bound by a …

The chapter about the Gram-positive bacterial cell wall gives a brief historical background
on the discovery of Gram-positive cell walls and their constituents and microscopic methods …

Bdellovibrio bacteriovorus is a microbial predator that offers promise as a living antibiotic for
its ability to kill Gram-negative bacteria, including human pathogens. Even after six decades …

Most, if not all, bacterial and archaeal cells contain at least one protein filament system.
Although these filament systems in some cases form structures that are very similar to …

Highlights•Cryo-ET and subtomogram averaging allow visualizing macromolecular
complexes in close to physiological conditions.•This offers a significant potential for in situ …

Biology spans a continuum of length and time scales. Individual experimental methods only
glimpse discrete pieces of this spectrum but can be combined to construct a more holistic …

The outer membrane (OM) of Gram-negative bacteria exhibits unique lipid asymmetry, with
lipopolysaccharides (LPS) residing in the outer leaflet and phospholipids (PLs) in the inner …

In most eukaryotic organisms, cilia and flagella perform a variety of life-sustaining roles
related to environmental sensing and motility. Cryo-electron microscopy has provided …