[HTML][HTML] Multiscale mechanobiology in brain physiology and diseases

A Procès, M Luciano, Y Kalukula, L Ris… - Frontiers in Cell and …, 2022 - frontiersin.org
Increasing evidence suggests that mechanics play a critical role in regulating brain function
at different scales. Downstream integration of mechanical inputs into biochemical signals …

Microtubule assembly: a review of progress, principles, and perspectives

DL Purich, D Kristofferson - Advances in protein chemistry, 1984 - Elsevier
Publisher Summary Substantial improvements in electron, light, and fluorescence
microscopy, as well as the isolation of discrete protein components of the cytoskeleton, have …

[HTML][HTML] Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.

JB Olmsted - Journal of Biological Chemistry, 1981 - Elsevier
A method is described for the affinity purification of antibodies using protein samples that
have been electrophoretically transferred to diazotized paper. Using differentiated …

A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs).

RB Vallee - The Journal of cell biology, 1982 - rupress.org
The effect of the antimitotic drug taxol on the association of MAPs (microtubule-associated
proteins) with microtubules was investigated. Extensive microtubule assembly occurred in …

Common and distinct tubulin binding sites for microtubule-associated proteins.

UZ Littauer, D Giveon, M Thierauf… - Proceedings of the …, 1986 - National Acad Sciences
A specific binding assay was developed that monitors the interaction of 125I-labeled
microtubule-associated proteins (MAPs) with tubulin or its fragments bound to nitrocellulose …

Analysis of MAP 4 function in living cells using green fluorescent protein (GFP) chimeras.

KR Olson, JR McIntosh, JB Olmsted - The Journal of cell biology, 1995 - rupress.org
MAP 4 is a ubiquitous microtubule-associated protein thought to play a role in the
polymerization and stability of microtubules in interphase and mitotic cells. We have …

A polymer-dependent increase in phosphorylation of beta-tubulin accompanies differentiation of a mouse neuroblastoma cell line.

DL Gard, MW Kirschner - The Journal of cell biology, 1985 - rupress.org
We have examined the phosphorylation of cellular microtubule proteins during
differentiation and neurite outgrowth in N115 mouse neuroblastoma cells. N115 …

Widespread distribution of the major polypeptide component of MAP 1 (microtubule-associated protein 1) in the nervous system.

GS Bloom, TA Schoenfeld, RB Vallee - The Journal of cell biology, 1984 - rupress.org
We prepared a monoclonal antibody to microtubule-associated protein 1 (MAP 1), one of the
two major high molecular weight MAP found in microtubules isolated from brain tissue. We …

Mitogen‐activated‐protein‐kinase‐catalyzed phosphorylation of microtubule‐associated proteins, microtubule‐associated protein 2 and microtubule‐associated …

M Hoshi, K Ohta, Y Gotoh, A Mori… - European Journal of …, 1992 - Wiley Online Library
Mitogen‐activated protein kinase (MAPK), a serine/threonine‐specific protein kinase which
is generally activated by stimulation with various growth factors and phorbol esters, utilizes …

[HTML][HTML] A model for microtubule-associated protein 4 structure. Domains defined by comparisons of human, mouse, and bovine sequences.

RR West, KM Tenbarge, JB Olmsted - Journal of Biological Chemistry, 1991 - Elsevier
cDNAs encoding human and mouse microtubule-associated protein 4 (MAP 4) were
isolated. MAP 4 is encoded by a single gene. Multiple MAP 4 mRNAs are transcribed that …