Novel insights into construct toxicity, strain optimization, and primary sequence design for producing recombinant silk fibroin and elastin-like peptide in E. coli
A Connor, C Wigham, Y Bai, M Rai, S Nassif… - Metabolic Engineering …, 2023 - Elsevier
Spider silk proteins (spidroins) are a remarkable class of biomaterials that exhibit a unique
combination of high-value attributes and can be processed into numerous morphologies for …
combination of high-value attributes and can be processed into numerous morphologies for …
Nanostructured, self-assembled spider silk materials for biomedical applications
The extraordinary mechanical properties of spider silk fibers result from the interplay of
composition, structure and self-assembly of spider silk proteins (spidroins). Genetic …
composition, structure and self-assembly of spider silk proteins (spidroins). Genetic …
Production and secretion of recombinant spider silk in Bacillus megaterium
Background Silk proteins have emerged as versatile biomaterials with unique chemical and
physical properties, making them appealing for various applications. Among them, spider …
physical properties, making them appealing for various applications. Among them, spider …
Formylation of recombinant spider silk in formic acid and wet spinning studied using nuclear magnetic resonance and infrared spectroscopies
T Asakura, H Matsuda, A Naito… - ACS Biomaterials Science …, 2022 - ACS Publications
We reported wet spinning of recombinant spider silk protein (RSSP) and formylation of
RSSP in formic acid (FA). First, FA was selected as the spinning solvent and the detailed …
RSSP in formic acid (FA). First, FA was selected as the spinning solvent and the detailed …
Recombinant spider silk fiber with high dimensional stability in water and its NMR characterization
T Asakura, H Matsuda, A Naito, H Okamura, Y Suzuki… - Molecules, 2022 - mdpi.com
Spider dragline silk has unique characteristics of strength and extensibility, including
supercontraction. When we use it as a biomaterial or material for textiles, it is important to …
supercontraction. When we use it as a biomaterial or material for textiles, it is important to …
Structural and Mechanical Variations of Major Ampullate Silk Spun by Malaysian Nephila pilipes Spider
Major ampullate (MA) silk is a natural biomaterial, spun by spiders with exceptional
mechanical characteristics. Most researchers who studied MA silk structure were primarily …
mechanical characteristics. Most researchers who studied MA silk structure were primarily …
Multispecies production of recombinant silk for future biomaterials manufacturing
A Connor - 2023 - search.proquest.com
As discussed in Chapter 1, work related to strain optimization, bioprocessing parameters,
and the identification of upstream production bottlenecks are sparse within the recombinant …
and the identification of upstream production bottlenecks are sparse within the recombinant …
Applicability of forcibly spinning technology to produce a finer silk filament
Y Kawahara, T Hananouchi - Journal of natural fibers, 2020 - Taylor & Francis
Forcibly spinning method has once shown a potential to enhance tensile strength of Bombyx
mori silkworm silk fibers to the level of spider dragline silk. Thus, the tensile data for the …
mori silkworm silk fibers to the level of spider dragline silk. Thus, the tensile data for the …
Squid suckerin-spider silk fusion protein hydrogel for secretome delivery to chronic wounds
KYH Koh - 2022 - dr.ntu.edu.sg
Chronic wounds are non-healing wounds characterized by a prolonged inflammation phase.
Excessive inflammation leads to elevated protease levels and consequently to a decrease in …
Excessive inflammation leads to elevated protease levels and consequently to a decrease in …