Spheroids and organoids are important novel players in medical and life science research.
They are gradually replacing two-dimensional (2D) cell cultures. Indeed, three-dimensional …

The liver is composed of two epithelial cell types: hepatocytes and liver ductal cells. Culture
conditions for expansion of human liver ductal cells in vitro as organoids were previously …

Breast cancer is characterized by histological and functional heterogeneity, posing a clinical
challenge for patient treatment. Emerging evidence suggests that the distinct subtypes …

High-grade serous ovarian cancer (HG-SOC)—often referred to as a “silent killer”—is the
most lethal gynecological malignancy. The fallopian tube (murine oviduct) and ovarian …

Cholangiocarcinoma (CCA) is a rare, aggressive disease with poor overall survival. In
advanced cases, surgery is often not possible or fails; in addition, there is a lack of effective …

Fibrolamellar carcinoma (FLC) is a lethal primary liver cancer, affecting young patients in
absence of chronic liver disease. Molecular understanding of FLC tumorigenesis is limited …

Organoids represent one of the most important advancements in the field of stem cells
during the past decade. They are three-dimensional in vitro culturing models that originate …

Organoid technology has rapidly transformed basic biomedical research and contributed to
significant discoveries in the last decade. With the application of protocols to generate …

Liver cancer and disease are among the most socially challenging global health concerns.
Although organ transplantation, surgical resection and anticancer drugs are the main …

Organoids derived from adult stem cells (ASCs) and pluripotent stem cells (PSCs) are
important preclinical models for studying cancer and developing therapies. Here, we review …