The manufacturing of recombinant protein is traditionally divided in two main steps:
upstream (cell culture and synthesis of the target protein) and downstream (purification and …

Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …

Today, potential immunogenicity can be better evaluated during the drug development
process, and we have rational approaches to manage the clinical consequences of …

Oxygen is a critical factor that can regulate the wound healing processes such as skin cell
proliferation, granulation, re-epithelialization, angiogenesis, and tissue regeneration …

Advances in nuclease-based gene-editing technologies have enabled precise, stable, and
systematic genetic engineering of glycosylation capacities in mammalian cells, opening up a …

Diatoms are marine organisms that represent one of the most important sources of biomass
in the ocean, accounting for about 40% of marine primary production, and in the biosphere …

Glycosylation patterns in antibodies critically determine biological and physical properties
but their precise control is a significant challenge in biology and biotechnology. We describe …

Mitigating risks to biotherapeutic protein production processes and products has driven the
development of targeted process analytical technology (PAT); however implementing PAT …

The application of process analytical technology (PAT) for biotherapeutic development and
manufacturing has been employed owing to technological, economic, and regulatory …

The success of clinical and commercial therapeutic proteins is rapidly increasing, but their
potential immunogenicity is an ongoing concern. Most of the studies that have been …