Split-inteins and their bioapplications

Y Li - Biotechnology letters, 2015 - Springer
Split-inteins are a subset of inteins that are expressed in two separate halves and catalyze
splicing in trans upon association of the two domains. They occur naturally and have also …

A dual ELP-tagged split intein system for non-chromatographic recombinant protein purification

C Shi, Q Meng, DW Wood - Applied microbiology and biotechnology, 2013 - Springer
Self-cleaving elastin-like protein (ELP) tags provide a very promising tool for recombinant
protein purification. With this method, the target protein is purified by simple ELP-mediated …

Protein Trans-Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins

Y Lin, M Li, H Song, L Xu, Q Meng, XQ Liu - PLoS One, 2013 - journals.plos.org
Protein trans-splicing by split inteins has many uses in protein production and research.
Splicing proteins with synthetic peptides, which employs atypical split inteins, is particularly …

Development of a screening system for inteins active in protein splicing based on intein insertion into the LacZα-peptide

M Neugebauer, JK Böcker, JCJ Matern… - Biological …, 2017 - degruyter.com
Protein splicing by inteins has found diverse applications in biotechnology, protein chemistry
and chemical biology. Inteins display a wide range of efficiencies and rates unpredictable …

Activation of Protease and Luciferase Using Engineered Nostoc punctiforme PCC73102 DnaE Intein with Altered Split Position

M Kawase, M Fujioka, T Takahashi - ChemBioChem, 2021 - Wiley Online Library
Inteins, self‐catalytic enzymes, have been widely used in the field of protein engineering
and chemical biology. Here, Nostoc punctiforme PCC73102 (Npu) DnaE intein was …

Site specific labeling of two proteins in one system by atypical split inteins

X Li, X Zhang, Y Cai, L Zhang, Y Lin, Q Meng - International journal of …, 2018 - Elsevier
Atypical inteins have been used for protein site-specific labeling and modification. S1 or S11
intein contain a small N-intein or C-intein which can be chemically synthesized and added …

Cysteine-free non-canonical C-intein for versatile protein C-terminal labeling through trans-splicing

X Dai, Q Xun, XQ Liu, Q Meng - Applied microbiology and biotechnology, 2015 - Springer
Site-specific protein labeling are powerful means of protein research and engineering;
however, new and improved labeling methods are greatly needed. Split inteins catalyze a …

Method of producing an immunoligand/payload conjugate

U Grawunder, RR Beerli - US Patent 11,986,535, 2024 - Google Patents
IEDXPSOJFSVCKU-HOKPPMCLSA-N [4-[[(2S)-5-(carbamoylamino)-2-[[(2S)-2-[6-(2, 5-
dioxopyrrolidin-1-yl) hexanoylamino]-3-methylbutanoyl] amino] pentanoyl] amino] phenyl] …

Binding protein drug conjugates comprising anthracycline derivatives

U Grawunder, RR Beerli - US Patent 11,833,120, 2023 - Google Patents
HKZAAJSTFUZYTO-LURJTMIESA-N (2s)-2-[[2-[[2-[[2-[(2-aminoacetyl) amino] acetyl] amino]
acetyl] amino] acetyl] amino]-3-hydroxypropanoic acid Chemical compound NCC (= O) NCC …

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