The crucial role of methodology development in directed evolution of selective enzymes
Directed evolution of stereo‐, regio‐, and chemoselective enzymes constitutes a unique way
to generate biocatalysts for synthetically interesting transformations in organic chemistry and …
to generate biocatalysts for synthetically interesting transformations in organic chemistry and …
Utility of B-factors in protein science: interpreting rigidity, flexibility, and internal motion and engineering thermostability
Z Sun, Q Liu, G Qu, Y Feng, MT Reetz - Chemical reviews, 2019 - ACS Publications
The term B-factor, sometimes called the Debye–Waller factor, temperature factor, or atomic
displacement parameter, is used in protein crystallography to describe the attenuation of X …
displacement parameter, is used in protein crystallography to describe the attenuation of X …
Single-sequence protein structure prediction using a language model and deep learning
R Chowdhury, N Bouatta, S Biswas, C Floristean… - Nature …, 2022 - nature.com
AlphaFold2 and related computational systems predict protein structure using deep learning
and co-evolutionary relationships encoded in multiple sequence alignments (MSAs) …
and co-evolutionary relationships encoded in multiple sequence alignments (MSAs) …
YASARA: a tool to obtain structural guidance in biocatalytic investigations
H Land, MS Humble - Protein engineering: methods and protocols, 2018 - Springer
In biocatalysis, structural knowledge regarding an enzyme and its substrate interactions
complements and guides experimental investigations. Structural knowledge regarding an …
complements and guides experimental investigations. Structural knowledge regarding an …
[HTML][HTML] Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently
The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …
ability to modify the sequence, and hence the structure and activity, of individual proteins in …
[HTML][HTML] Carbonic anhydrase to boost CO2 sequestration: Improving carbon capture utilization and storage (CCUS)
A de Oliveira Maciel, P Christakopoulos, U Rova… - Chemosphere, 2022 - Elsevier
Abstract CO 2 Capture Utilization and Storage (CCUS) is a fundamental strategy to mitigate
climate change, and carbon sequestration, through absorption, can be one of the solutions …
climate change, and carbon sequestration, through absorption, can be one of the solutions …
[HTML][HTML] Rational design of enzyme activity and enantioselectivity
The strategy of rational design to engineer enzymes is to predict the potential mutants based
on the understanding of the relationships between protein structure and function, and …
on the understanding of the relationships between protein structure and function, and …
Enabling broader adoption of biocatalysis in organic chemistry
EO Romero, AT Saucedo, JR Hernández-Meléndez… - JACS Au, 2023 - ACS Publications
Biocatalysis is becoming an increasingly impactful method in contemporary synthetic
chemistry for target molecule synthesis. The selectivity imparted by enzymes has been …
chemistry for target molecule synthesis. The selectivity imparted by enzymes has been …
[HTML][HTML] Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications
Competitive sustainable production in industry demands new and better biocatalysts,
optimized bioprocesses and cost-effective product recovery. Our review sheds light on the …
optimized bioprocesses and cost-effective product recovery. Our review sheds light on the …
Enzyme engineering: current trends and future perspectives
Enzyme-based biocatalysts have a wide range of industrial applications for production,
processing and improving the quality attributes of animal feed, beverages, detergent, food …
processing and improving the quality attributes of animal feed, beverages, detergent, food …