The role of FACT in managing chromatin: disruption, assembly, or repair?
Abstract FACT (FAcilitates Chromatin Transcription) has long been considered to be a
transcription elongation factor whose ability to destabilize nucleosomes promotes RNAPII …
transcription elongation factor whose ability to destabilize nucleosomes promotes RNAPII …
Functional consequences of bidirectional promoters
Several studies have shown that promoters of protein-coding genes are origins of pervasive
non-coding RNA transcription and can initiate transcription in both directions. However, only …
non-coding RNA transcription and can initiate transcription in both directions. However, only …
[HTML][HTML] Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis
Maintaining proper mRNA levels is a key aspect in the regulation of gene expression. The
balance between mRNA synthesis and decay determines these levels. We demonstrate that …
balance between mRNA synthesis and decay determines these levels. We demonstrate that …
Dynamic transcriptome analysis measures rates of mRNA synthesis and decay in yeast
To obtain rates of mRNA synthesis and decay in yeast, we established dynamic
transcriptome analysis (DTA). DTA combines non‐perturbing metabolic RNA labeling with …
transcriptome analysis (DTA). DTA combines non‐perturbing metabolic RNA labeling with …
The role of FACT in making and breaking nucleosomes
T Formosa - Biochimica Et Biophysica Acta (BBA)-Gene Regulatory …, 2012 - Elsevier
FACT is a roughly 180kDa heterodimeric protein complex important for managing the
properties of chromatin in eukaryotic cells. Chromatin is a repressive barrier that plays an …
properties of chromatin in eukaryotic cells. Chromatin is a repressive barrier that plays an …
[HTML][HTML] A complete set of nascent transcription rates for yeast genes
The amount of mRNA in a cell is the result of two opposite reactions: transcription and
mRNA degradation. These reactions are governed by kinetics laws, and the most regulated …
mRNA degradation. These reactions are governed by kinetics laws, and the most regulated …
[HTML][HTML] Promoter elements regulate cytoplasmic mRNA decay
A Bregman, M Avraham-Kelbert, O Barkai, L Duek… - Cell, 2011 - cell.com
Promoters are DNA elements that enable transcription and its regulation by trans-acting
factors. Here, we demonstrate that yeast promoters can also regulate mRNA decay after the …
factors. Here, we demonstrate that yeast promoters can also regulate mRNA decay after the …
[HTML][HTML] Long-distance cooperative and antagonistic RNA polymerase dynamics via DNA supercoiling
Genes are often transcribed by multiple RNA polymerases (RNAPs) at densities that can
vary widely across genes and environmental conditions. Here, we provide in vitro and in …
vary widely across genes and environmental conditions. Here, we provide in vitro and in …
Feedback regulation of ribosome assembly
J de la Cruz, F Gómez-Herreros, O Rodríguez-Galán… - Current genetics, 2018 - Springer
Ribosome biogenesis is a crucial process for growth and constitutes the major consumer of
cellular resources. This pathway is subjected to very stringent regulation to ensure correct …
cellular resources. This pathway is subjected to very stringent regulation to ensure correct …
[HTML][HTML] Engineered covalent inactivation of TFIIH-kinase reveals an elongation checkpoint and results in widespread mRNA stabilization
JB Rodríguez-Molina, SC Tseng, SP Simonett… - Molecular cell, 2016 - cell.com
During transcription initiation, the TFIIH-kinase Kin28/Cdk7 marks RNA polymerase II (Pol II)
by phosphorylating the C-terminal domain (CTD) of its largest subunit. Here we describe a …
by phosphorylating the C-terminal domain (CTD) of its largest subunit. Here we describe a …