The ability to control cell volume is pivotal for cell function. Cell volume perturbation elicits a
wide array of signaling events, leading to protective (eg, cytoskeletal rearrangement) and …

Lang, Florian, Gillian L. Busch, Markus Ritter, Harald Völkl, Siegfried Waldegger, Erich
Gulbins, and Dieter Häussinger. Functional Significance of Cell Volume Regulatory …

Simplex optimization has generated several media that have improved the development of
mouse preimplantation embryos in vitro. One objective of this study was to compare the …

We have recently reported the use of sequential simplex optimization methods to design a
medium (SOM) that overcomes the block to development beyond two cells which occurs in …

Mammalian cells utilize a wide variety of cell volume regulatory mechanisms. For rapid
adjustment of cell volume cells release or accumulate ions through respective channels and …

We have previously indicated that porcine blastocysts can be produced by in vitro
fertilization (IVF) and culture (IVC) in chemically defined porcine gamete medium (PGM) and …

In this review, 4 areas of embryo research are discussed and their relevance to mammalian
embryo culture is considered. The areas are:(1) perturbation of embryonic development by …

The success of clinical IVF was initially compromised by sub-optimal culture conditions,
resulting in impaired embryo development (1–6) and a subsequent loss of viability …

Cell shrinkage is a hallmark of the apoptotic mode of programmed cell death, but it is as yet
unclear whether a reduction in cell volume is a primary activation signal of apoptosis. Here …

Mitogens control progression through the cell cycle in non-transformed cells by complex
cascades of intracellular messengers, such as Ca 2+ and protons, and by cell volume …