Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology
For centuries, cannabis has been a rich source of fibrous, pharmaceutical, and recreational
ingredients. Phytocannabinoids are the most important and well-known class of cannabis …
ingredients. Phytocannabinoids are the most important and well-known class of cannabis …
Genetic engineering meets hematopoietic stem cell biology for next-generation gene therapy
The growing clinical success of hematopoietic stem/progenitor cell (HSPC) gene therapy
(GT) relies on the development of viral vectors as portable" Trojan horses" for safe and …
(GT) relies on the development of viral vectors as portable" Trojan horses" for safe and …
Human genetic diversity alters off-target outcomes of therapeutic gene editing
CRISPR gene editing holds great promise to modify DNA sequences in somatic cells to treat
disease. However, standard computational and biochemical methods to predict off-target …
disease. However, standard computational and biochemical methods to predict off-target …
CRISPR-based therapeutics: current challenges and future applications
The discovery, only a decade ago, of the genome editing power of clustered regularly
interspaced short palindromic repeats (CRISPR)-associated nucleases is already …
interspaced short palindromic repeats (CRISPR)-associated nucleases is already …
[HTML][HTML] Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression
Background Chimeric antigen receptor (CAR) T cells have demonstrated high clinical
response rates against hematological malignancies (eg, CD19+ cancers) but have shown …
response rates against hematological malignancies (eg, CD19+ cancers) but have shown …
Benchmarking deep learning methods for predicting CRISPR/Cas9 sgRNA on-and off-target activities
G Zhang, Y Luo, X Dai, Z Dai - Briefings in Bioinformatics, 2023 - academic.oup.com
In silico design of single guide RNA (sgRNA) plays a critical role in clustered regularly
interspaced, short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) …
interspaced, short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) …
[HTML][HTML] CRISPR-based bioengineering in microalgae for production of industrially important biomolecules
D Dhokane, A Shaikh, A Yadav, N Giri… - … in Bioengineering and …, 2023 - frontiersin.org
Microalgae, as photosynthetic organisms, have the potential to produce biomolecules for
use in food, feed, cosmetics, nutraceuticals, fuel, and other applications. Faster growth rates …
use in food, feed, cosmetics, nutraceuticals, fuel, and other applications. Faster growth rates …
Functional annotation of lncRNA in high-throughput screening
Recent efforts on the characterization of long non-coding RNAs (lncRNAs) revealed their
functional roles in modulating diverse cellular processes. These include pluripotency …
functional roles in modulating diverse cellular processes. These include pluripotency …
Novel strategies in transplantation: genetic engineering and vascularized composite allotransplantation
M Kauke-Navarro, OF Noel, L Knoedler… - Journal of Surgical …, 2023 - Elsevier
Introduction Despite the clinical success in vascularized composite allotransplantation
(VCA), systemic immunosuppression remains necessary to prevent allograft rejection. Even …
(VCA), systemic immunosuppression remains necessary to prevent allograft rejection. Even …
[HTML][HTML] Advancements in CRISPR screens for the development of cancer immunotherapy strategies
CRISPR screen technology enables systematic and scalable interrogation of gene function
by using the CRISPR-Cas9 system to perturb gene expression. In the field of cancer …
by using the CRISPR-Cas9 system to perturb gene expression. In the field of cancer …